CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012032
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DDX10 
Protein Synonyms/Alias
 DEAD box protein 10 
Gene Name
 DDX10 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
62RLMQNYEKINVNEITubiquitination[1, 2]
79SDFPLSKKTLKGLQEubiquitination[2]
97RLVTEIQKQTIGLALubiquitination[2]
107IGLALQGKDVLGAAKubiquitination[2]
114KDVLGAAKTGSGKTLubiquitination[2]
269DLARLSLKNPEYVWVubiquitination[2]
410AGRTARYKEDGEALLubiquitination[2]
434VQQLLQKKVPVKEIKubiquitination[2]
453KLIDVQKKLESILAQacetylation[3]
453KLIDVQKKLESILAQubiquitination[2]
465LAQDQDLKERAQRCFubiquitination[2]
515PRVRFLQKMQKQPTKubiquitination[2]
522KMQKQPTKELVRSQAubiquitination[2]
657DEKTLQKKEPSKSSIubiquitination[4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Putative ATP-dependent RNA helicase. 
Sequence Annotation
 DOMAIN 100 274 Helicase ATP-binding.
 DOMAIN 287 448 Helicase C-terminal.
 NP_BIND 89 91 ATP.
 NP_BIND 113 120 ATP.
 MOTIF 69 97 Q motif.
 MOTIF 222 225 DEAD box.
 BINDING 96 96 ATP.
 MOD_RES 4 4 Phosphothreonine.
 MOD_RES 539 539 Phosphoserine.
 MOD_RES 780 780 Phosphoserine.
 MOD_RES 831 831 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 875 AA 
Protein Sequence
MGKTANSPGS GARPDPVRSF NRWKKKHSHR QNKKKQLRKQ LKKPEWQVER ESISRLMQNY 60
EKINVNEITR FSDFPLSKKT LKGLQEAQYR LVTEIQKQTI GLALQGKDVL GAAKTGSGKT 120
LAFLVPVLEA LYRLQWTSTD GLGVLIISPT RELAYQTFEV LRKVGKNHDF SAGLIIGGKD 180
LKHEAERINN INILVCTPGR LLQHMDETVS FHATDLQMLV LDEADRILDM GFADTMNAVI 240
ENLPKKRQTL LFSATQTKSV KDLARLSLKN PEYVWVHEKA KYSTPATLEQ NYIVCELQQK 300
ISVLYSFLRS HLKKKSIVFF SSCKEVQYLY RVFCRLRPGV SILALHGRQQ QMRRMEVYNE 360
FVRKRAAVLF ATDIAARGLD FPAVNWVLQF DCPEDANTYI HRAGRTARYK EDGEALLILL 420
PSEKAMVQQL LQKKVPVKEI KINPEKLIDV QKKLESILAQ DQDLKERAQR CFVSYVRSVY 480
LMKDKEVFDV SKLPIPEYAL SLGLAVAPRV RFLQKMQKQP TKELVRSQAD KVIEPRAPSL 540
TNDEVEEFRA YFNEKMSILQ KGGKRLEGTE HRQDNDTGNE EQEEEEDDEE EMEEKLAKAK 600
GSQAPSLPNT SEAQKIKEVP TQFLDRDEEE EDADFLKVKR HNVFGLDLKD EKTLQKKEPS 660
KSSIKKKMTK VAEAKKVMKR NFKVNKKITF TDEGELVQQW PQMQKSAIKD AEEDDDTGGI 720
NLHKAKERLQ EEDKFDKEEY RKKIKAKHRE KRLKEREARR EANKRQAKAK DEEEAFLDWS 780
DDDDDDDDGF DPSTLPDPDK YRSSEDSDSE DMENKISDTK KKQGMKKRSN SEVEDVGPTS 840
HNRKKARWDT LEPLDTGLSL AEDEELVLHL LRSQS 875 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; TAS:ProtInc. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR025313; DUF4217.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF13959; DUF4217
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS