UniProt Accession

 PMYT1_HUMAN; Q99640; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2; O14731; Q7LE24; Q8TCM9 

Genbank Protein ID

 AF014118; U56816; AK097642; AK098452; AK301926; AF549406; AC004233; AC004235; CH471112; CH471112; AL713779 

Genbank Nucleotide ID

 AAB71843.1; AAC50949.1; BAG53500.1; BAG63346.1; AAN40703.1; AAC04478.1; AAC04477.1; EAW85439.1; EAW85440.1; CAD28540.1 

Protein Name

 Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase 

Protein Synonyms/Alias

 Myt1 kinase 

Gene Name

 PKMYT1 

Gene Synonyms/Alias

 MYT1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
126	GSYGEVFKVRSKEDG	ubiquitination	[1]
154	GPKDRARKLAEVGSH	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. 

Sequence Annotation

 DOMAIN 110 359 Protein kinase.
 NP_BIND 116 124 ATP (By similarity).
 REGION 398 499 Interaction with PIN1.
 REGION 437 499 Interaction with CDC2-CCNB1.
 MOTIF 382 398 Membrane-association motif.
 ACT_SITE 233 233 Proton acceptor (By similarity).
 METAL 238 238 Magnesium; via carbonyl oxygen (By
 METAL 251 251 Magnesium; via carbonyl oxygen (By
 BINDING 139 139 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 17 17 Phosphothreonine.
 MOD_RES 94 94 Phosphoserine.
 MOD_RES 120 120 Phosphoserine.
 MOD_RES 160 160 Phosphoserine.
 MOD_RES 426 426 Phosphoserine; by PLK1.
 MOD_RES 469 469 Phosphoserine.
 MOD_RES 473 473 Phosphoserine.
 MOD_RES 495 495 Phosphothreonine; by PLK1.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell cycle; Complete proteome; Endoplasmic reticulum; Golgi apparatus; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 499 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; TAS:ProtInc.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; TAS:ProtInc.
 GO:0007088; P:regulation of mitosis; TAS:ProtInc. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS.
 IPR016235; Tyr/Thr_kinase_Cdc2_inhib. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS