CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012443
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-CoA synthetase short-chain family member 3, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Acss3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
123IENGQGDKIAIIYDSacetylation[1]
210STRIDHAKPKVVVTAacetylation[1, 2]
257YSRPNMEKVPLMSGRubiquitination[3]
414QYSLTRFKTLFVAGEacetylation[1]
513GAFSGLWKNQEAFKHacetylation[4]
513GAFSGLWKNQEAFKHsuccinylation[4]
519WKNQEAFKHLYFEKFacetylation[1, 2, 5]
601LALCVLKKDVNASEEacetylation[5]
616QVLEEIVKHVRQSIGacetylation[1]
657LSALVNGKPYKVTPTacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753
Functional Description
 Activates acetate so that it can be used for lipid synthesis or for energy generation (By similarity). 
Sequence Annotation
  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 682 AA 
Protein Sequence
MKPSWLQCRK VTGAGTLGAP LPGSPSVRGA AVTRRALVAG FGGRGCRALT TGSGGEYKTH 60
FAASVADPER FWGKAAEQIS WYKPWTKTLE SRYPPSTSWF VEGMLNICYN AIDRHIENGQ 120
GDKIAIIYDS PVTDTKATIS YKEVLEQVSK LAGVLVKQGV KKGDTVVIYM PMIPQAIYTM 180
LACARIGAIH SLIFGGFASK ELSTRIDHAK PKVVVTASFG IEPGRKVEYI PLLEEALRIG 240
QHRPDRVLIY SRPNMEKVPL MSGRDLDWEE EMAKAQSHDC VPVLSEHPLY ILYTSGTTGL 300
PKGVVRPTGG YAVMLNWTMS SIYGLKPGEV WWAASDLGWV VGHSYICYGP LLHGNTTVLY 360
EGKPVGTPDA GAYFRVLAEH GVAALFTAPT AIRAIRQQDP GAALGKQYSL TRFKTLFVAG 420
ERCDVETLEW SKKVFRVPVL DHWWQTETGS PITASCIGLG NSKTPPPGQA GKCVPGYNVM 480
ILDDNMQKLK ARSLGNIVVK LPLPPGAFSG LWKNQEAFKH LYFEKFPGYY DTMDAGYMDE 540
EGYLYVMSRV DDVINVAGHR ISAGAIEESV LSHGTVADCA VVGKEDPLKG HVPLALCVLK 600
KDVNASEEQV LEEIVKHVRQ SIGPVAAFRN AVFVKQLPKT RSGKIPRSTL SALVNGKPYK 660
VTPTIEDPSI FGHIEEVLKQ AV 682 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003987; F:acetate-CoA ligase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW. 
Interpro
 IPR024597; Acyl-CoA_synth_DUF3448.
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig.
 IPR025110; DUF4009. 
Pfam
 PF00501; AMP-binding
 PF11930; DUF3448
 PF13193; DUF4009 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS