CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031286
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosolic acyl coenzyme A thioester hydrolase 
Protein Synonyms/Alias
 cDNA FLJ60167, highly similar to Cytosolic acyl coenzyme A thioester hydrolase(EC 3.1.2.2) 
Gene Name
 ACOT7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42GAKKLTNKATLWYVPacetylation[1]
42GAKKLTNKATLWYVPubiquitination[2]
53WYVPLSLKNVDKVLEacetylation[3, 4]
53WYVPLSLKNVDKVLEubiquitination[2, 4, 5, 6, 7, 8]
57LSLKNVDKVLEVPPVacetylation[1, 4]
57LSLKNVDKVLEVPPVubiquitination[7]
83RKRYEAQKLERMETKacetylation[4]
83RKRYEAQKLERMETKubiquitination[4, 7]
90KLERMETKWRNGDIVubiquitination[7]
152IVAARHCKTNIVTASubiquitination[7]
168DAINFHDKIRKGCVIacetylation[1, 4]
168DAINFHDKIRKGCVIubiquitination[2, 4, 7]
171NFHDKIRKGCVITISubiquitination[4, 7]
206PVVDSSQKRYRAASAubiquitination[9]
254KGRYLQMKAKRQGHAubiquitination[7]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 265 AA 
Protein Sequence
MCIGEVAHVS AEITYTSKHS VEVQVNVMSE NILTGAKKLT NKATLWYVPL SLKNVDKVLE 60
VPPVVYSRQE QEEEGRKRYE AQKLERMETK WRNGDIVQPV LNPEPNTVSY SQSSLIHLVG 120
PSDCTLHGFV HGGVTMKLMD EVAGIVAARH CKTNIVTASV DAINFHDKIR KGCVITISGR 180
MTFTSNKSME IEVLVDADPV VDSSQKRYRA ASAFFTYVSL SQEGRSLPVP QLVPETEDEK 240
KRFEEGKGRY LQMKAKRQGH AEPQP 265 
Gene Ontology
 GO:0044297; C:cell body; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:Compara.
 GO:0009062; P:fatty acid catabolic process; IEA:Compara. 
Interpro
 IPR006683; Thioestr_supf. 
Pfam
 PF03061; 4HBT 
SMART
  
PROSITE
  
PRINTS