UniProt Accession

 LYS1_YEAST; P38998; D6VVW5 

Genbank Protein ID

 X77362; Z38061; BK006942 

Genbank Nucleotide ID

 CAA54551.1; CAA86194.1; DAA08581.1 

Protein Name

 Saccharopine dehydrogenase [NAD(+), L-lysine-forming] 

Protein Synonyms/Alias

 SDH; Lysine--2-oxoglutarate reductase 

Gene Name

 LYS1 

Gene Synonyms/Alias

 YIR034C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
13	LHLRAETKPLEARAA	ubiquitination	[1]
173	VSPYPNEKALVKDVT	acetylation	[2]
348	LELLPQRKTAPVWVR	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the NAD(+)-dependent cleavage of saccharopine to L-lysine and 2-oxoglutarate. 

Sequence Annotation

 ACT_SITE 205 205
 MOD_RES 2 2 N-acetylalanine; partial.  

Keyword

 3D-structure; Acetylation; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Lysine biosynthesis; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 373 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0003729; F:mRNA binding; IDA:SGD.
 GO:0004754; F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity; IDA:SGD.
 GO:0019878; P:lysine biosynthetic process via aminoadipic acid; NAS:SGD. 

Interpro

 IPR007886; AlaDH/PNT_N.
 IPR007698; AlaDH/PNT_NAD(H)-bd.
 IPR027281; Lys1. 

Pfam

 PF01262; AlaDh_PNT_C
 PF05222; AlaDh_PNT_N 

SMART

 SM01002; AlaDh_PNT_C
 SM01003; AlaDh_PNT_N 

PROSITE

  

PRINTS