UniProt Accession

 FHP_YEAST; P39676; D6VV14; Q6B1W6; Q9C1R6 

Genbank Protein ID

 L07070; L07071; X87941; Z73019; AY692964; AF239759; BK006941 

Genbank Nucleotide ID

 CAA61184.1; CAA97262.1; AAT92983.1; AAK15081.1; DAA08325.1 

Protein Name

 Flavohemoprotein 

Protein Synonyms/Alias

 Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD 

Gene Name

 YHB1 

Gene Synonyms/Alias

 YHB; YGR234W; G8572 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
66	TTVLAAAKNIDDLSV	ubiquitination	[1]
217	SLCSASTKNGLRFAV	ubiquitination	[1]
225	NGLRFAVKMEAAREN	acetylation	[2]
350	LINAAFLKEKSPAHA	acetylation	[2]
350	LINAAFLKEKSPAHA	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the fungus from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress. 

Sequence Annotation

 DOMAIN 147 264 FAD-binding FR-type.
 NP_BIND 207 210 FAD (By similarity).
 NP_BIND 281 286 NADP (By similarity).
 NP_BIND 389 392 FAD (By similarity).
 REGION 1 138 Globin.
 REGION 146 399 Reductase.
 REGION 272 399 NAD or NADP-binding.
 ACT_SITE 95 95 Charge relay system (By similarity).
 ACT_SITE 137 137 Charge relay system (By similarity).
 METAL 85 85 Iron (heme proximal ligand) (By
 BINDING 189 189 FAD (By similarity).
 MOD_RES 22 22 Phosphothreonine.  

Keyword

 Complete proteome; Cytoplasm; Detoxification; Direct protein sequencing; FAD; Flavoprotein; Heme; Iron; Metal-binding; NAD; NADP; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 399 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005759; C:mitochondrial matrix; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008941; F:nitric oxide dioxygenase activity; IEA:EC.
 GO:0016966; F:nitric oxide reductase activity; IMP:SGD.
 GO:0019825; F:oxygen binding; IEA:InterPro.
 GO:0015671; P:oxygen transport; IEA:InterPro.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR000971; Globin.
 IPR009050; Globin-like.
 IPR012292; Globin_dom.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00042; Globin
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR
 PS01033; GLOBIN 

PRINTS