CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020939
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 pre-rRNA processing protein FTSJ3 
Protein Synonyms/Alias
 2'-O-ribose RNA methyltransferase SPB1 homolog; Protein ftsJ homolog 3; Putative rRNA methyltransferase 3 
Gene Name
 Ftsj3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
157RGGCFITKVFRSRDYacetylation[1]
214DAKFFDPKFAFKEVEubiquitination[2]
233TVTELVTKKKPKAEGacetylation[1, 3]
634VKRGRGSKADEDGFEacetylation[3]
650VPIQDPVKYRILDPEubiquitination[2]
669GAVIASSKKAKRDLIubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation (By similarity). 
Sequence Annotation
 ACT_SITE 157 157 Proton acceptor (By similarity).
 BINDING 56 56 S-adenosyl-L-methionine; via amide
 BINDING 58 58 S-adenosyl-L-methionine; via amide
 BINDING 76 76 S-adenosyl-L-methionine (By similarity).
 BINDING 92 92 S-adenosyl-L-methionine (By similarity).
 BINDING 117 117 S-adenosyl-L-methionine (By similarity).
 MOD_RES 333 333 Phosphoserine.
 MOD_RES 335 335 Phosphoserine.
 MOD_RES 336 336 Phosphoserine.
 MOD_RES 369 369 Phosphothreonine (By similarity).
 MOD_RES 545 545 Phosphoserine (By similarity).
 MOD_RES 576 576 Phosphoserine (By similarity).
 MOD_RES 667 667 Phosphoserine (By similarity).
 MOD_RES 679 679 Phosphoserine (By similarity).  
Keyword
 Coiled coil; Complete proteome; Direct protein sequencing; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 838 AA 
Protein Sequence
MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD LCAAPGGWLQ 60
VAAKFMPVSS LIVGVDLVPI KPLPNVVTLQ EDITTERCRQ ALRKELKTWK VDVVLNDGAP 120
NVGASWVHDA YSQAHLTLMA LRLACDFLAR GGCFITKVFR SRDYQPLLWI FQQLFHRVQA 180
TKPQASRHES AEIFVVCQGF LAPDKVDAKF FDPKFAFKEV EVQAKTVTEL VTKKKPKAEG 240
YAEGDLTLYH RTSVTDFLRA ANPVDFLSKA SEISIDDEEL AQHPATTEDI RVCCQDIKVL 300
GRKELRSLLN WRTKLRRYVA KKLKEQAKAL DISLSSEEEE EGDEEEAVAE TKQAPEEEEE 360
REEEQLNRTL AEMKAQEVAE LKRKKKKLLR EQRKQRERVE LKMDLPGVSI ADEGETGMFS 420
LRTIRGQQLL EEVTQGDMNA ADTFLSDLPR DDIYVSDAED DDDTSLESDL DPEELAGVRT 480
HSDLKEQKYL RFTQVDDNKE EEGENPLLVP LEEKAVLQEE QASLWFSKDG FSGIEDDADE 540
ALEISQAQLL YKSRQKEQQP TDPPPPPTNL KTEKKSPQGQ NEVPKETEAI LGTEAVTDPG 600
GEERGNSSDS DSSSSEDEDS WKVSRGVKRG RGSKADEDGF EVVPIQDPVK YRILDPEGLA 660
LGAVIASSKK AKRDLIDNSF NRYAFNEEEG ELPEWFAQEE KQHRIRQLPV DKKEVEHYRK 720
RWREINARPI KKVAEAKARK KRRVLKKLEQ TKKKAEAVVN TVDISEREKV AQLRSLYKKA 780
GLGKEKRQVT YVVAKKGVGR KVRRPAGVKG HFKVVDSRMK KDQRAQQRKE QKKKHKRK 838 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0001510; P:RNA methylation; IEA:InterPro.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR015507; rRNA-MeTfrase_E.
 IPR012920; rRNA_MeTfrase_Spb1_C.
 IPR024576; rRNA_MeTfrase_Spb1_DUF3381.
 IPR002877; rRNA_MeTrfase_FtsJ_dom. 
Pfam
 PF11861; DUF3381
 PF01728; FtsJ
 PF07780; Spb1_C 
SMART
  
PROSITE
  
PRINTS