CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007589
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Condensin-2 complex subunit D3 
Protein Synonyms/Alias
 Non-SMC condensin II complex subunit D3; hCAP-D3 
Gene Name
 NCAPD3 
Gene Synonyms/Alias
 CAPD3; KIAA0056 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
148DKCIQTLKKSWPQESubiquitination[1]
149KCIQTLKKSWPQESNubiquitination[1, 2]
442HQKFLKHKFLVQEIMubiquitination[1]
456MFDRCLDKAPTVRSKubiquitination[1]
559DEKTNVRKSALQVLVubiquitination[1]
578HCDVSGMKEDLWILQubiquitination[1]
597DPAVSVRKQALQSLTubiquitination[1]
618PRCVQIQKAWLRGVVubiquitination[1, 2]
687ELSRYLNKAFHIWSKubiquitination[1]
724PAWMLLSKIAGSSPRubiquitination[1]
743RIIQSWEKISSQQNPubiquitination[1]
776LPKSTRDKVTDAVKCubiquitination[1]
782DKVTDAVKCKLNGFQubiquitination[1]
841RLSNIVLKENGTGNMubiquitination[1]
929HAIITLGKLCLQHEDubiquitination[1]
940QHEDLAKKSIPALVRubiquitination[1]
1074EKHEKYNKFPQSEREubiquitination[1]
1088EKRLFSLKGKSNKERubiquitination[1]
1155VLSSKEIKLLAMRSKubiquitination[1]
1162KLLAMRSKPDKDLLMubiquitination[1]
1165AMRSKPDKDLLMEEDubiquitination[1]
1187VMQEAQKKLISQVQKubiquitination[1]
1194KLISQVQKRNFIENIubiquitination[1]
1246KDFFAVDKQLASELEubiquitination[3]
1257SELEYDMKKYQEQLVubiquitination[3]
1258ELEYDMKKYQEQLVQubiquitination[1, 3, 4, 5]
1360AILNSVKKAVESKSRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. 
Sequence Annotation
 REPEAT 441 475 HEAT 1.
 REPEAT 537 572 HEAT 2.
 REPEAT 579 610 HEAT 3.
 REPEAT 973 1009 HEAT 4.
 MOD_RES 567 567 Phosphoserine.
 MOD_RES 1329 1329 Phosphoserine.
 MOD_RES 1331 1331 Phosphothreonine.
 MOD_RES 1348 1348 Phosphoserine.
 MOD_RES 1357 1357 Phosphoserine.
 MOD_RES 1379 1379 Phosphothreonine.
 MOD_RES 1382 1382 Phosphoserine.
 MOD_RES 1384 1384 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Coiled coil; Complete proteome; DNA condensation; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1498 AA 
Protein Sequence
MVALRGLGSG LQPWCPLDLR LEWVDTVWEL DFTETEPLDP SIEAEIIETG LAAFTKLYES 60
LLPFATGEHG SMESIWTFFI ENNVSHSTLV ALFYHFVQIV HKKNVSVQYR EYGLHAAGLY 120
FLLLEVPGSV ANQVFHPVMF DKCIQTLKKS WPQESNLNRK RKKEQPKSSQ ANPGRHRKRG 180
KPPRREDIEM DEIIEEQEDE NICFSARDLS QIRNAIFHLL KNFLRLLPKF SLKEKPQCVQ 240
NCIEVFVSLT NFEPVLHECH VTQARALNQA KYIPELAYYG LYLLCSPIHG EGDKVISCVF 300
HQMLSVILML EVGEGSHRAP LAVTSQVINC RNQAVQFISA LVDELKESIF PVVRILLQHI 360
CAKVVDKSEY RTFAAQSLVQ LLSKLPCGEY AMFIAWLYKY SRSSKIPHRV FTLDVVLALL 420
ELPEREVDNT LSLEHQKFLK HKFLVQEIMF DRCLDKAPTV RSKALSSFAH CLELTVTSAS 480
ESILELLINS PTFSVIESHP GTLLRNSSAF SYQRQTSNRS EPSGEINIDS SGETVGSGER 540
CVMAMLRRRI RDEKTNVRKS ALQVLVSILK HCDVSGMKED LWILQDQCRD PAVSVRKQAL 600
QSLTELLMAQ PRCVQIQKAW LRGVVPVVMD CESTVQEKAL EFLDQLLLQN IRHHSHFHSG 660
DDSQVLAWAL LTLLTTESQE LSRYLNKAFH IWSKKEKFSP TFINNVISHT GTEHSAPAWM 720
LLSKIAGSSP RLDYSRIIQS WEKISSQQNP NSNTLGHILC VIGHIAKHLP KSTRDKVTDA 780
VKCKLNGFQW SLEVISSAVD ALQRLCRASA ETPAEEQELL TQVCGDVLST CEHRLSNIVL 840
KENGTGNMDE DLLVKYIFTL GDIAQLCPAR VEKRIFLLIQ SVLASSADAD HSPSSQGSSE 900
APASQPPPQV RGSVMPSVIR AHAIITLGKL CLQHEDLAKK SIPALVRELE VCEDVAVRNN 960
VIIVMCDLCI RYTIMVDKYI PNISMCLKDS DPFIRKQTLI LLTNLLQEEF VKWKGSLFFR 1020
FVSTLIDSHP DIASFGEFCL AHLLLKRNPV MFFQHFIECI FHFNNYEKHE KYNKFPQSER 1080
EKRLFSLKGK SNKERRMKIY KFLLEHFTDE QRFNITSKIC LSILACFADG ILPLDLDASE 1140
LLSDTFEVLS SKEIKLLAMR SKPDKDLLME EDDMALANVV MQEAQKKLIS QVQKRNFIEN 1200
IIPIIISLKT VLEKNKIPAL RELMHYLREV MQDYRDELKD FFAVDKQLAS ELEYDMKKYQ 1260
EQLVQEQELA KHADVAGTAG GAEVAPVAQV ALCLETVPVP AGQENPAMSP AVSQPCTPRA 1320
SAGHVAVSSP TPETGPLQRL LPKARPMSLS TIAILNSVKK AVESKSRHRS RSLGVLPFTL 1380
NSGSPEKTCS QVSSYSLEQE SNGEIEHVTK RAISTPEKSI SDVTFGAGVS YIGTPRTPSS 1440
AKEKIEGRSQ GNDILCLSLP DKPPPQPQQW NVRSPARNKD TPACSRRSLR KTPLKTAN 1498 
Gene Ontology
 GO:0031618; C:nuclear centromeric heterochromatin; IDA:BHF-UCL.
 GO:0000799; C:nuclear condensin complex; IDA:UniProtKB.
 GO:0035064; F:methylated histone residue binding; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007076; P:mitotic chromosome condensation; IDA:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR026971; CND1/NCAPD3.
 IPR026003; Cohesin_HEAT.
 IPR012371; NCAPD3. 
Pfam
 PF12765; Cohesin_HEAT 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS