CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018089
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 4 regulatory subunit 1 
Protein Synonyms/Alias
  
Gene Name
 PPP4R1 
Gene Synonyms/Alias
 MEG1; PP4R1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53TPLGRLDKYAASENIubiquitination[1]
612PDEERRTKVQDVVPQubiquitination[1, 2]
643TVDTEIAKHCAYSLPubiquitination[1, 3, 4]
730KHLHDFLKLLHIDKRubiquitination[5, 6, 7]
803SVRWISYKLVSEMVKubiquitination[3, 5, 7]
887NVRVLLAKTLRQTLLubiquitination[1, 4]
937SIHPASTKISEDAMSubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. 
Sequence Annotation
 REPEAT 1 25 HEAT 1.
 REPEAT 26 63 HEAT 2.
 REPEAT 65 81 HEAT 3.
 REPEAT 82 119 HEAT 4.
 REPEAT 127 164 HEAT 5.
 REPEAT 168 206 HEAT 6.
 REPEAT 208 246 HEAT 7.
 REPEAT 248 285 HEAT 8.
 REPEAT 287 324 HEAT 9.
 REPEAT 505 542 HEAT 10.
 REPEAT 568 606 HEAT 11.
 REPEAT 698 734 HEAT 12.
 REPEAT 799 837 HEAT 13.
 REPEAT 861 898 HEAT 14.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 950 AA 
Protein Sequence
MADLSLLQED LQEDADGFGV DDYSSESDVI IIPSALDFVS QDEMLTPLGR LDKYAASENI 60
FNRQMVARSL LDTLREVCDD ERDCIAVLER ISRLADDSEP TVRAELMEQV PHIALFCQEN 120
RPSIPYAFSK FLLPIVVRYL ADQNNQVRKT SQAALLALLE QELIERFDVE TKVCPVLIEL 180
TAPDSNDDVK TEAVAIMCKM APMVGKDITE RLILPRFCEM CCDCRMFHVR KVCAANFGDI 240
CSVVGQQATE EMLLPRFFQL CSDNVWGVRK ACAECFMAVS CATCQEIRRT KLSALFINLI 300
SDPSRWVRQA AFQSLGPFIS TFANPSSSGQ YFKEESKSSE EMSVENKNRT RDQEAPEDVQ 360
VRPEDTPSDL SVSNSSVILE NTMEDHAAEA SGKPLGEISV PLDSSLLCTL SSESHQEAAS 420
NENDKKPGNY KSMLRPEVGT TSQDSALLDQ ELYNSFHFWR TPLPEIDLDI ELEQNSGGKP 480
SPEGPEEESE GPVPSSPNIT MATRKELEEM IENLEPHIDD PDVKAQVEVL SAALRASSLD 540
AHEETISIEK RSDLQDELDI NELPNCKINQ EDSVPLISDA VENMDSTLHY IHSDSDLSNN 600
SSFSPDEERR TKVQDVVPQA LLDQYLSMTD PSRAQTVDTE IAKHCAYSLP GVALTLGRQN 660
WHCLRETYET LASDMQWKVR RTLAFSIHEL AVILGDQLTA ADLVPIFNGF LKDLDEVRIG 720
VLKHLHDFLK LLHIDKRREY LYQLQEFLVT DNSRNWRFRA ELAEQLILLL ELYSPRDVYD 780
YLRPIALNLC ADKVSSVRWI SYKLVSEMVK KLHAATPPTF GVDLINELVE NFGRCPKWSG 840
RQAFVFVCQT VIEDDCLPMD QFAVHLMPHL LTLANDRVPN VRVLLAKTLR QTLLEKDYFL 900
ASASCHQEAV EQTIMALQMD RDSDVKYFAS IHPASTKISE DAMSTASSTY 950 
Gene Ontology
 GO:0030289; C:protein phosphatase 4 complex; ISS:UniProtKB.
 GO:0030362; F:protein phosphatase type 4 regulator activity; NAS:UniProtKB.
 GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
 GO:0007165; P:signal transduction; NAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000357; HEAT.
 IPR021133; HEAT_type_2. 
Pfam
 PF02985; HEAT 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS