CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Focadhesin 
Protein Synonyms/Alias
  
Gene Name
 FOCAD 
Gene Synonyms/Alias
 KIAA1797 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37KENGFSEKIHQSTNQubiquitination[1]
54ALNLLWEKCCSDNVVubiquitination[1, 2]
122KEGQGGEKNIQSIYTubiquitination[1, 2, 3]
343LSVTEDQKIPKSSLLubiquitination[1, 3]
399CYRDDHQKLSYKLVCubiquitination[2]
553LLTSLWEKQDRVYPEubiquitination[1, 2]
577VPSLSVGKEVQWEKLubiquitination[1, 2, 3, 4]
583GKEVQWEKLIAKAASubiquitination[1, 2, 3, 5, 6, 7]
587QWEKLIAKAASIRDIubiquitination[1, 2, 3]
654TWNALSPKLSCDTRPubiquitination[1]
799GIYHSALKGGARSDQubiquitination[1]
808GARSDQGKTVAGIPNubiquitination[1, 2]
819GIPNFILKMYETNKQacetylation[8]
930PEEVQYKKSTAWLWVubiquitination[1, 4]
947MLTDEITKAAAKESPubiquitination[1]
951EITKAAAKESPVVKGubiquitination[3]
1168HVAALLRKLSAHVDDubiquitination[1]
1254KAEDLGSKLLPAWIRubiquitination[3]
1576NRIAQVTKSNIEKAAubiquitination[3, 9]
1639HANTGVLKRMEWLLEubiquitination[3]
1777AQSRDLLKATLLSLRubiquitination[1, 5, 7, 9]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Potential tumor suppressor in gliomas (By similarity). 
Sequence Annotation
 MOD_RES 819 819 N6-acetyllysine.  
Keyword
 Acetylation; Cell junction; Complete proteome; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1801 AA 
Protein Sequence
MSDDIRKRFE FPNSLIQSQA VGHLIAAVLK ENGFSEKIHQ STNQTPALNL LWEKCCSDNV 60
VVRTACCEGL VALVAQDHAE FSYVLNGILN LIPSTRNTHG LIKAIMHLLQ MQALKEGQGG 120
EKNIQSIYTI RNHPHPLITV LEHRPDCWPV FLQQLTAFFQ QCPERLEVSC IQIMAPFLWY 180
LYCEPSQLQE YAKLRLALLK VLLQPQVLCD KDQPSILEQQ ILQLCCDIVP CLQVKDLIQT 240
TEAMMFIEEV CLSLLRHPVF WKIQLTQMSL QLLCVSEVSL KITGECSSSI HLLEHSVELL 300
KEDFPVELVI IGIALLLLQT PASQQKPILN LALKLLSVTE DQKIPKSSLL LVMPILQILS 360
STALEDCISV DEEGPSRQQL ALNLLEMIQQ ECYRDDHQKL SYKLVCPVTS MYGTIFTAWR 420
ILEVMTDSSA ASDWLASVES LLPITAVIPA PAFLLLAHLL VEDKGQNLHQ ILKVTTELAQ 480
ADSSQVPNLI PVLMFKLGRP LEPILYNDIL YTLPKLGVHK VCIGQILRII QLLGTTPRLR 540
AVTLRLLTSL WEKQDRVYPE LQRFMAVSDV PSLSVGKEVQ WEKLIAKAAS IRDICKQRPY 600
QHGADMLAAI SQVLNECTKP DQATPAALVL QGLHALCQAE VVCIRSTWNA LSPKLSCDTR 660
PLILKTLSEL FSLVPSLTVN TTEYENFKVQ VLSFLWTHTQ NKDPIVANAA YRSLANFTAG 720
EHTILHLPEK IRPEIPIPEE LDDDEDVEDV DLSVPGSCYL KLLSLTPPLV LPALEEFFTS 780
LVKQEMVNMP RGIYHSALKG GARSDQGKTV AGIPNFILKM YETNKQPGLK PGLAGGMLFC 840
YDVSMYQSKD GKPLNRLMAS RGRSFKQTSL ALVHEVHIQL SEWHRAIFLP QAWLAYMNRA 900
YHAILQGRLG ELELQLKHGK EEPEEVQYKK STAWLWVRDM LTDEITKAAA KESPVVKGNA 960
LLALSSLAVV VSRHEASLSS DSDGLLEVQP NFLSMKEWVS MVLDTLLVIV DSHYQPRGQL 1020
LSWFYYKSYS GENTASAIAR SAAATALSLL VPVFIISCKE KVEEILNMLT ARLPGKPSAD 1080
ESQAVQIHMG LALGMFLSRL CEEKLSDISG QEMNLLLMKS LDALENCCFD TSLEYNTGCI 1140
LGVGLVLSLM SHSSQMQSRV HVAALLRKLS AHVDDSGSQS RTFQEVLAYT LSCVCTSAFS 1200
AGIIEATEAE DVMNKLRLLV ENSQQTSGFA LALGNIVHGL SVCGHGKAED LGSKLLPAWI 1260
RIVLTEGTPT MLCLAALHGM VALVGSEGDV MQLKSEAIQT SHFQGRLNEV IRTLTQVISV 1320
SGVIGLQSNA VWLLGHLHLS TLSSSQSRAS VPTDYSYLPE SSFIGAAIGF FITGGKKGPE 1380
SVPPSLLKVV MKPIATVGES YQYPPVNWAA LLSPLMRLNF GEEIQQLCLE IMVTQAQSSQ 1440
NAAALLGLWV TPPLIHSLSL NTKRYLLISA PLWIKHISDE QILGFVENLM VAVFKAASPL 1500
GSPELCPSAL HGLSQAMKLP SPAHHLWSLL SEATGKIFDL LPNKIRRKDL ELYISIAKCL 1560
LEMTDDDANR IAQVTKSNIE KAAFVKLYLV SQGRFPLVNL TDMLSVAVQH REKEVLAWMI 1620
LHSLYQARIV SHANTGVLKR MEWLLELMGY IRNVAYQSTS FHNTALDKAL DFFLLIFATA 1680
VVAWADHTAP LLLGLSASWL PWHQENGPAG PVPSFLGRSP MHRVTLQEVL TLLPNSMALL 1740
LQKEPWKEQT QKFIDWLFSI MESPKEALSA QSRDLLKATL LSLRVLPEFK KKAVWTRAYG 1800
W 1801 
Gene Ontology
 GO:0005925; C:focal adhesion; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR021392; DUF3028.
 IPR022542; DUF3730. 
Pfam
 PF11229; DUF3028
 PF12530; DUF3730 
SMART
  
PROSITE
  
PRINTS