CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023640
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Afadin- and alpha-actinin-binding protein 
Protein Synonyms/Alias
 ADIP; Afadin DIL domain-interacting protein; SSX2-interacting protein 
Gene Name
 SSX2IP 
Gene Synonyms/Alias
 KIAA0923 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
112ELLVLQRKNLLAQENubiquitination[1, 2, 3, 4]
126NVETQNLKLGSDMDHubiquitination[3]
355ILKSHVEKLDNQVSKubiquitination[3]
362KLDNQVSKVHLEGFNubiquitination[2]
398QQCKEMIKTQQQLLQubiquitination[2]
429DCYLLEEKERLKEEWubiquitination[3, 5]
476EERASWLKQQFLNMTubiquitination[1, 2, 4, 5]
535VCMSKLTKSLPASPSubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E- cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). 
Sequence Annotation
 MOD_RES 290 290 Phosphoserine.
 MOD_RES 293 293 Phosphoserine.
 MOD_RES 312 312 Phosphoserine.
 MOD_RES 536 536 Phosphoserine.  
Keyword
 Alternative splicing; Cell adhesion; Cell junction; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 614 AA 
Protein Sequence
MGDWMTVTDP GLSSESKTIS QYTSETKMSP SSLYSQQVLC SSIPLSKNVH SFFSAFCTED 60
NIEQSISYLD QELTTFGFPS LYEESKGKET KRELNIVAVL NCMNELLVLQ RKNLLAQENV 120
ETQNLKLGSD MDHLQSCYSK LKEQLETSRR EMIGLQERDR QLQCKNRNLH QLLKNEKDEV 180
QKLQNIIASR ATQYNHDMKR KEREYNKLKE RLHQLVMNKK DKKIAMDILN YVGRADGKRG 240
SWRTGKTEAR NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR 300
ERVDDSTGTV ISDVEEDAGE LSRESMWDLS CETVREQLTN SIRKQWRILK SHVEKLDNQV 360
SKVHLEGFND EDVISRQDHE QETEKLELEI QQCKEMIKTQ QQLLQQQLAT AYDDDTTSLL 420
RDCYLLEEKE RLKEEWSLFK EQKKNFERER RSFTEAAIRL GLERKAFEEE RASWLKQQFL 480
NMTTFDHQNS ENVKLFSAFS GSSDWDNLIV HSRQPQKKPH SVSNGSPVCM SKLTKSLPAS 540
PSTSDFCQTR SCISEHSSIN VLNITAEEIK PNQVGGECTN QKWSVASRPG SQEGCYSGCS 600
LSYTNSHVEK DDLP 614 
Gene Ontology
 GO:0005913; C:cell-cell adherens junction; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IDA:LIFEdb.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW. 
Interpro
 IPR021622; Afadin/alpha-actinin-bd. 
Pfam
 PF11559; ADIP 
SMART
  
PROSITE
  
PRINTS