CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002716
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Abhydrolase domain-containing protein 2 
Protein Synonyms/Alias
 Lung alpha/beta hydrolase 2; Protein PHPS1-2 
Gene Name
 ABHD2 
Gene Synonyms/Alias
 LABH2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36IVRCLNLKSPTAPPDubiquitination[1, 2]
57GLSRFLLKSCPLLTKubiquitination[1, 2, 3, 4, 5]
64KSCPLLTKEYIPPLIubiquitination[2]
74IPPLIWGKSGHIQTAubiquitination[2]
85IQTALYGKMGRVRSPubiquitination[1, 2]
152TFVDYAQKNGYRCAVubiquitination[1, 2, 3, 5, 6]
194AMVNYIKKTYPLTQLubiquitination[1]
215LGGNIVCKYLGETQAubiquitination[1]
265FLMADNMKKIILSHRubiquitination[1, 2, 6]
266LMADNMKKIILSHRQubiquitination[2]
281ALFGDHVKKPQSLEDubiquitination[2, 7]
282LFGDHVKKPQSLEDTubiquitination[1, 2, 6]
310IDDNVMRKFHGYNSLubiquitination[8]
318FHGYNSLKEYYEEESubiquitination[8]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 May play a role in smooth muscle cells migration (By similarity). 
Sequence Annotation
 ACT_SITE 207 207 Charge relay system (By similarity).
 ACT_SITE 345 345 Charge relay system (By similarity).
 ACT_SITE 376 376 Charge relay system (By similarity).  
Keyword
 Complete proteome; Hydrolase; Membrane; Polymorphism; Reference proteome; Serine esterase; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 425 AA 
Protein Sequence
MNAMLETPEL PAVFDGVKLA AVAAVLYVIV RCLNLKSPTA PPDLYFQDSG LSRFLLKSCP 60
LLTKEYIPPL IWGKSGHIQT ALYGKMGRVR SPHPYGHRKF ITMSDGATST FDLFEPLAEH 120
CVGDDITMVI CPGIANHSEK QYIRTFVDYA QKNGYRCAVL NHLGALPNIE LTSPRMFTYG 180
CTWEFGAMVN YIKKTYPLTQ LVVVGFSLGG NIVCKYLGET QANQEKVLCC VSVCQGYSAL 240
RAQETFMQWD QCRRFYNFLM ADNMKKIILS HRQALFGDHV KKPQSLEDTD LSRLYTATSL 300
MQIDDNVMRK FHGYNSLKEY YEEESCMRYL HRIYVPLMLV NAADDPLVHE SLLTIPKSLS 360
EKRENVMFVL PLHGGHLGFF EGSVLFPEPL TWMDKLVVEY ANAICQWERN KLQCSDTEQV 420
EADLE 425 
Gene Ontology
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0030336; P:negative regulation of cell migration; IEA:Compara.
 GO:0009611; P:response to wounding; IEA:Compara. 
Interpro
 IPR012020; AB-Hydro_YheT.
 IPR000073; AB_hydrolase_1.
 IPR000952; UPF0017_hydro-like_CS. 
Pfam
 PF00561; Abhydrolase_1 
SMART
  
PROSITE
 PS01133; UPF0017 
PRINTS