UniProt Accession

 CISY_MOUSE; Q9CZU6; Q3UDP3 

Genbank Protein ID

 AB056479; AK012151; AK145643; AK149990; AK163273; AK166814; AK167125; BC013554; BC029754 

Genbank Nucleotide ID

 BAB63945.1; BAB28063.1; BAE26560.1; BAE29218.1; BAE37268.1; BAE39041.1; BAE39273.1; AAH13554.1; AAH29754.1 

Protein Name

 Citrate synthase, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Cs 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
34	SASSTNLKDVLSNLI	acetylation	[1]
43	VLSNLIPKEQARIKT	acetylation	[1, 2, 3, 4, 5, 6]
43	VLSNLIPKEQARIKT	ubiquitination	[7]
49	PKEQARIKTFKQQHG	acetylation	[1, 4]
52	QARIKTFKQQHGKTV	acetylation	[1, 2, 3, 4, 6]
57	TFKQQHGKTVVGQIT	acetylation	[1, 8]
57	TFKQQHGKTVVGQIT	succinylation	[8]
76	YGGMRGMKGLVYETS	acetylation	[4]
76	YGGMRGMKGLVYETS	ubiquitination	[7]
103	YSIPECQKMLPKAKG	acetylation	[2, 4, 5, 6, 8]
103	YSIPECQKMLPKAKG	succinylation	[8]
103	YSIPECQKMLPKAKG	ubiquitination	[7]
193	AEGMNRAKYWELIYE	acetylation	[8]
193	AEGMNRAKYWELIYE	succinylation	[8]
215	KLPCVAAKIYRNLYR	ubiquitination	[7]
321	QLQKEVGKDVSDEKL	acetylation	[1, 2, 4, 6, 8]
321	QLQKEVGKDVSDEKL	succinylation	[8]
327	GKDVSDEKLRDYIWN	acetylation	[1, 2, 4, 6, 8, 9]
327	GKDVSDEKLRDYIWN	succinylation	[8]
366	CQREFALKHLPKDPM	acetylation	[4]
370	FALKHLPKDPMFKLV	acetylation	[1, 4]
370	FALKHLPKDPMFKLV	ubiquitination	[7]
375	LPKDPMFKLVAQLYK	acetylation	[1, 2, 3, 4, 6, 8, 9, 10]
375	LPKDPMFKLVAQLYK	succinylation	[8]
382	KLVAQLYKIVPNILL	acetylation	[1, 2, 3, 5]
393	NILLEQGKAKNPWPN	acetylation	[1, 2, 3, 4, 6, 8]
393	NILLEQGKAKNPWPN	succinylation	[8]
393	NILLEQGKAKNPWPN	ubiquitination	[7]
450	GFPLERPKSMSTDGL	acetylation	[1, 4, 5, 8]
450	GFPLERPKSMSTDGL	succinylation	[8]
450	GFPLERPKSMSTDGL	ubiquitination	[7]
459	MSTDGLMKFVDSK**	acetylation	[1, 3, 4, 5, 6, 8]
459	MSTDGLMKFVDSK**	succinylation	[8]
459	MSTDGLMKFVDSK**	ubiquitination	[7]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [9] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [10] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 

Functional Description

  

Sequence Annotation

 ACT_SITE 301 301 By similarity.
 ACT_SITE 347 347 By similarity.
 ACT_SITE 402 402 By similarity.
 MOD_RES 76 76 N6-acetyllysine; alternate (By
 MOD_RES 76 76 N6-succinyllysine; alternate (By
 MOD_RES 327 327 N6-acetyllysine (By similarity).
 MOD_RES 375 375 N6-acetyllysine (By similarity).
 MOD_RES 382 382 N6-acetyllysine (By similarity).
 MOD_RES 393 393 N6-acetyllysine (By similarity).
 MOD_RES 395 395 N6,N6,N6-trimethyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Methylation; Mitochondrion; Reference proteome; Transferase; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 464 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
 GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway. 

Interpro

 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR010109; Citrate_synthase_euk. 

Pfam

 PF00285; Citrate_synt 

SMART

  

PROSITE

 PS00480; CITRATE_SYNTHASE 

PRINTS

 PR00143; CITRTSNTHASE.