CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030591
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP30 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
170QQSEITPKQITCRTRubiquitination[1]
258VVCDNCTKIEAKGTLubiquitination[1, 2]
269KGTLNGEKVEHQRTTubiquitination[1]
279HQRTTFVKQLKLGKLubiquitination[1]
307SSHGTPLKRHEHVQFubiquitination[1]
341QHNPKLNKNPGPTLEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MKNWGVIGGI AAALAAGIYV IWGPITERKK RRKGLVPGLV NLGNTCFMNS LLQGLSACPA 60
FIRWLEEFTS QYSRDQKEPP SHQYLSLTLL HLLKALSCQE VTDDEVLDAS CLLDVLRMYR 120
WQISSFEEQD AHELFHVITS SLEDERDRQP RVTHLFDVHS LEQQSEITPK QITCRTRGSP 180
HPTSNHWKSQ HPFHGRLTSN MVCKHCEHQS PVRFDTFDSL SLSIPAATWG HPLTLDHCLH 240
HFISSESVRD VVCDNCTKIE AKGTLNGEKV EHQRTTFVKQ LKLGKLPQCL CIHLQRLSWS 300
SHGTPLKRHE HVQFNEFLMM DIYKYHLLGH KPSQHNPKLN KNPGPTLELQ DGPGAPTPVL 360
NQPGAPKTQI FMNGACSPSL LPTLSAPMPF PLPVVPDYSS STYLFRLMAV VVHHGDMHSG 420
HFVTYRRSPP SARNPLSTSN QWLWVSDDTV RKASLQEVLS SSAYLLFYER VLSRMQHQSQ 480
ECKSEE 486 
Gene Ontology
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS