CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022874
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 282 
Protein Synonyms/Alias
 HTLV-I U5RE-binding protein 1; HUB-1 
Gene Name
 ZNF282 
Gene Synonyms/Alias
 HUB1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111AIQAVERKVDAQASQubiquitination[1]
190LRLPPGSKGEAPKVPubiquitination[2]
228ELYNNLVKENYKTLMubiquitination[2, 3, 4]
232NLVKENYKTLMSLDAubiquitination[2]
293QDASSQVKREDTLCVubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat. It recognizes the 5'-TCCACCCC-3' sequence as a core motif and exerts a strong repressive effect on HTLV-I LTR-mediated expression. 
Sequence Annotation
 DOMAIN 198 271 KRAB.
 ZN_FING 518 540 C2H2-type 1.
 ZN_FING 546 568 C2H2-type 2.
 ZN_FING 574 596 C2H2-type 3.
 ZN_FING 602 624 C2H2-type 4.
 ZN_FING 630 652 C2H2-type 5.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 671 AA 
Protein Sequence
MQFVSTRPQP QQLGIQGLGL DSGSWSWAQA LPPEEVCHQE PALRGEMAEG MPPMQAQEWD 60
MDARRPMPFQ FPPFPDRAPV FPDRMMREPQ LPTAEISLWT VVAAIQAVER KVDAQASQLL 120
NLEGRTGTAE KKLADCEKTA VEFGNHMESK WAVLGTLLQE YGLLQRRLEN LENLLRNRNF 180
WVLRLPPGSK GEAPKVPVTF VDIAVYFSED EWKNLDEWQK ELYNNLVKEN YKTLMSLDAE 240
GSVPKPDAPV QAEPREEPCV WEQRHPEERE IPMDPEAGAE PLVPAQDASS QVKREDTLCV 300
RGQRGLEERA IPTESITDSP ISAQDLLSRI KQEEHQCVWD QQDLADRDIP TDPNSESLIS 360
AHDILSWIKQ EEQPYPWGPR DSMDGELGLD SGPSDSLLMV KNPPPAPPQP QPQPQPPQPQ 420
LQSQPQPQSL PPIAVAENPG GPPSRGLLDD GFQVLPGERG SGEAPPGGDR STGGGGGDGG 480
GGGGGAEAGT GAGGGCGSCC PGGLRRSLLL HGARSKPYSC PECGKSFGVR KSLIIHHRSH 540
TKERPYECAE CEKSFNCHSG LIRHQMTHRG ERPYKCSECE KTYSRKEHLQ NHQRLHTGER 600
PFQCALCGKS FIRKQNLLKH QRIHTGERPY TCGECGKSFR YKESLKDHLR VHSGGPGPGA 660
PRQLPPPPER D 671 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; TAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR022137; DUF3669_Znf.
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF12417; DUF3669
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS