UniProt Accession

 CNN2_HUMAN; Q99439; A5D8U8; A6NFI4; D6W5X9; Q92578 

Genbank Protein ID

 D83735; AK126391; AC004528; AC011558; CH471139; CH471139; CH471139; BC141818; BC141833; D86059 

Genbank Nucleotide ID

 BAA12090.1; BAG54322.1; EAW69565.1; EAW69567.1; EAW69570.1; AAI41819.1; AAI41834.1; BAA20887.1 

Protein Name

 Calponin-2 

Protein Synonyms/Alias

 Calponin H2, smooth muscle; Neutral calponin 

Gene Name

 CNN2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MSSTQFNKGPSYGLS	acetylation	[1]
25	VKNRLLSKYDPQKEA	acetylation	[1]
25	VKNRLLSKYDPQKEA	ubiquitination	[2]
174	GLQMGTNKCASQSGM	ubiquitination	[3]
234	RRHIYDTKLGTDKCD	ubiquitination	[3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. 

Sequence Annotation

 DOMAIN 28 131 CH.
 REPEAT 166 191 Calponin-like 1.
 REPEAT 206 231 Calponin-like 2.
 REPEAT 245 269 Calponin-like 3.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 8 8 N6-acetyllysine.
 MOD_RES 25 25 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Actin-binding; Alternative splicing; Calmodulin-binding; Complete proteome; Direct protein sequencing; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 309 AA 

Protein Sequence

Gene Ontology

 GO:0005911; C:cell-cell junction; TAS:ProtInc.
 GO:0001725; C:stress fiber; IDA:MGI.
 GO:0003779; F:actin binding; TAS:ProtInc.
 GO:0031032; P:actomyosin structure organization; IEA:InterPro.
 GO:0071260; P:cellular response to mechanical stimulus; IDA:MGI.
 GO:0007010; P:cytoskeleton organization; TAS:ProtInc.
 GO:0030097; P:hemopoiesis; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; IEA:Compara.
 GO:0050765; P:negative regulation of phagocytosis; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0032970; P:regulation of actin filament-based process; IDA:MGI.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0042060; P:wound healing; IEA:Compara. 

Interpro

 IPR001997; Calponin.
 IPR000557; Calponin_repeat.
 IPR001715; CH-domain.
 IPR003096; SM22_calponin. 

Pfam

 PF00402; Calponin
 PF00307; CH 

SMART

 SM00033; CH 

PROSITE

 PS01052; CALPONIN_1
 PS51122; CALPONIN_2
 PS50021; CH 

PRINTS

 PR00889; CALPONIN.
 PR00888; SM22CALPONIN.