CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018858
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Osteoclast-stimulating factor 1 
Protein Synonyms/Alias
  
Gene Name
 OSTF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82NPLHEAAKRGNLSWLubiquitination[1]
162IVQLLLAKGARTDLRubiquitination[1, 2, 3, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity. 
Sequence Annotation
 DOMAIN 12 71 SH3.
 REPEAT 72 101 ANK 1.
 REPEAT 105 135 ANK 2.
 REPEAT 139 168 ANK 3.
 MOD_RES 200 200 Phosphothreonine.
 MOD_RES 202 202 Phosphoserine.
 MOD_RES 213 213 Phosphoserine.  
Keyword
 3D-structure; ANK repeat; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 214 AA 
Protein Sequence
MSKPPPKPVK PGQVKVFRAL YTFEPRTPDE LYFEEGDIIY ITDMSDTNWW KGTSKGRTGL 60
IPSNYVAEQA ESIDNPLHEA AKRGNLSWLR ECLDNRVGVN GLDKAGSTAL YWACHGGHKD 120
IVEMLFTQPN IELNQQNKLG DTALHAAAWK GYADIVQLLL AKGARTDLRN IEKKLAFDMA 180
TNAACASLLK KKQGTDAVRT LSNAEDYLDD EDSD 214 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; TAS:ProtInc.
 GO:0001503; P:ossification; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR000108; p67phox.
 IPR001452; SH3_domain. 
Pfam
 PF12796; Ank_2
 PF00018; SH3_1 
SMART
 SM00248; ANK
 SM00326; SH3 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50002; SH3 
PRINTS
 PR01415; ANKYRIN.
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.