UniProt Accession

 KEFF_ECOLI; P0A754; P31577; P75629 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC73157.1; BAB96614.2 

Protein Name

 Glutathione-regulated potassium-efflux system ancillary protein KefF 

Protein Synonyms/Alias

 Quinone oxidoreductase KefF 

Gene Name

 kefF 

Gene Synonyms/Alias

 yabF; b0046; JW0045 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
80	LLKLWIDKVFSHGWA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC. Can use a wide range of substrates, including electrophilic quinones, and its function could be to reduce the redox toxicity of electrophilic quinones in parallel with acting as triggers for the KefC efflux system. 

Sequence Annotation

 NP_BIND 14 17 FMN.
 NP_BIND 65 68 FMN.
 NP_BIND 105 108 FMN.
 BINDING 8 8 FMN.  

Keyword

 3D-structure; Cell inner membrane; Cell membrane; Complete proteome; Flavoprotein; FMN; Membrane; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 176 AA 

Protein Sequence

Gene Ontology

 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0009055; F:electron carrier activity; IMP:EcoliWiki.
 GO:0010181; F:FMN binding; IDA:EcoCyc.
 GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IDA:EcoCyc.
 GO:0032414; P:positive regulation of ion transmembrane transporter activity; IDA:EcoCyc.
 GO:0006813; P:potassium ion transport; IEA:InterPro. 

Interpro

 IPR003680; Flavodoxin_fold.
 IPR023948; K_H_efflux_KefF. 

Pfam

 PF02525; Flavodoxin_2 

SMART

  

PROSITE

  

PRINTS