| Tag | Content |
|---|
| CPLM ID | CPLM-010390 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA topoisomerase 1 |
Protein Synonyms/Alias | DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme |
Gene Name | topA |
Gene Synonyms/Alias | MPN_261; MP572 |
Created Date | July 27, 2013 |
Organism | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
NCBI Taxa ID | 272634 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 69 | KFSSLSFKGNLKGKK | acetylation | [1] | | 85 | LSKYDIIKSIKEKAS | acetylation | [1] | | 249 | PALRAGLKFEEEKSV | acetylation | [1] | | 367 | ILNHYGEKYLSNRIV | acetylation | [1] | | 446 | RFINDGNKFYASSKS | acetylation | [1] | | 572 | IINKEFTKNMEKSLD | acetylation | [1] | | 604 | SNFKEEVKLAEGSIQ | acetylation | [1] | | 642 | YTKRGNEKFVGCSNF | acetylation | [1] | | 686 | QLVKRRTKFNPNKTF | acetylation | [1] | | 691 | RTKFNPNKTFVGCSN | acetylation | [1] |
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Reference | [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium. van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC. Mol Syst Biol. 2012 Feb 28;8:571. [ PMID: 22373819] |
Functional Description | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). |
Sequence Annotation | DOMAIN 3 134 Toprim.
ZN_FING 624 652 C4-type 1.
ZN_FING 673 702 C4-type 2.
REGION 183 188 Interaction with DNA (By similarity).
ACT_SITE 340 340 O-(5'-phospho-DNA)-tyrosine intermediate
METAL 9 9 Magnesium 1; catalytic (By similarity).
METAL 102 102 Magnesium 1; catalytic (By similarity).
METAL 102 102 Magnesium 2 (By similarity).
METAL 104 104 Magnesium 2 (By similarity). |
Keyword | ATP-binding; Complete proteome; DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Repeat; Topoisomerase; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 711 AA |
Protein Sequence | MSKNLVVIES PNKVKTLQKY LPNDFEIVST IGHIREMVHK NFGFNEADYS PVWEDWTKSK 60
KKFSSLSFKG NLKGKKLLSK YDIIKSIKEK ASKATNIYLA TDPDREGEAI SWHVYDVLDE 120
KDKSKCQRIT FNEITKNAVL DALKNPREID QSWVQSQFAR QILDRMIGFR LSRLLNNYLS 180
AKSAGRVQSV ALRFLEEREQ EIRSFVPRFW WTLDVLLNPK AEGVREACAN RSIPIVLREI 240
NPALRAGLKF EEEKSVSGID FLDEASAKKF GEQLKGVFEV YNIDETKHYS SSPNSAYTTA 300
SLQKDAINKL GWSSKKVTLI AQHLYEGVSI NGEQTALISY PRTDSTRLSA QFQQSCKEYI 360
LNHYGEKYLS NRIVSAKGKK GEKIIQDAHE AIHPTDINIT PEMVKNAIKK DEFLLYRLIW 420
IRTVASLMAD CKKSHTHIRF INDGNKFYAS SKSLVFDGYR KIYEHFENKE SNDLYIDLDK 480
IRVGDRFMAK DIKITARQTH PAARYTQASL IEALEKSNIG RPSTYNTMAS VNLDRGYASL 540
NKHAFHVTQL GEQVNEELSK HFGKIINKEF TKNMEKSLDE IAENKKNYQE FLRDFWSNFK 600
EEVKLAEGSI QRVKKEKEFV GRDCPSCASP LLYRYTKRGN EKFVGCSNFP NCKYNEFSQN 660
KPNLTLEKLE ELCPECNSQL VKRRTKFNPN KTFVGCSNFP RCRYIKKDNA S 711 |
Gene Ontology | |
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Pfam | |
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