CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011972
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 ECH1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83NKRNAMNKVFWREMVacetylation[1]
83NKRNAMNKVFWREMVubiquitination[2]
95EMVECFNKISRDADCacetylation[1]
214GTLQRLPKVIGNQSLubiquitination[3, 4]
231ELAFTARKMMADEALubiquitination[2]
276PVAVQSTKVNLLYSRubiquitination[2, 5]
318VQATTENKELKTVTFacetylation[1, 6]
318VQATTENKELKTVTFubiquitination[2, 6]
327LKTVTFSKL******acetylation[1]
327LKTVTFSKL******ubiquitination[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA (By similarity). 
Sequence Annotation
 MOTIF 326 328 Microbody targeting signal (Potential).
 BINDING 174 174 Substrate; via amide nitrogen (By
 MOD_RES 327 327 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Isomerase; Lipid metabolism; Mitochondrion; Peroxisome; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 328 AA 
Protein Sequence
MAAGIVASRR LRDLLTRRLT GSNYPGLSIS LRLTGSSAQE EASGVALGEA PDHSYESLRV 60
TSAQKHVLHV QLNRPNKRNA MNKVFWREMV ECFNKISRDA DCRAVVISGA GKMFTAGIDL 120
MDMASDILQP KGDDVARISW YLRDIITRYQ ETFNVIERCP KPVIAAVHGG CIGGGVDLVT 180
ACDIRYCAQD AFFQVKEVDV GLAADVGTLQ RLPKVIGNQS LVNELAFTAR KMMADEALGS 240
GLVSRVFPDK EVMLDAALAL AAEISSKSPV AVQSTKVNLL YSRDHSVAES LNYVASWNMS 300
MLQTQDLVKS VQATTENKEL KTVTFSKL 328 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005777; C:peroxisome; NAS:UniProtKB.
 GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR014748; Crontonase_C.
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 
Pfam
 PF00378; ECH 
SMART
  
PROSITE
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS