CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021121
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 7 
Protein Synonyms/Alias
 Beta-Pix; PAK-interacting exchange factor beta; p85SPR 
Gene Name
 Arhgef7 
Gene Synonyms/Alias
 Kiaa0142; Pak3bp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
55LLPGTIEKVYPEPRNubiquitination[1]
384VLTTGLSKPFMRLDKubiquitination[1]
794KIIVEETKSNGQTVIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity). 
Sequence Annotation
 DOMAIN 1 111 CH.
 DOMAIN 163 222 SH3.
 DOMAIN 250 430 DH.
 DOMAIN 452 557 PH.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 164 164 Phosphoserine.
 MOD_RES 228 228 Phosphoserine (By similarity).
 MOD_RES 497 497 Phosphoserine.
 MOD_RES 673 673 Phosphoserine; by CaMK1 (By similarity).
 MOD_RES 776 776 Phosphoserine.  
Keyword
 3D-structure; Alternative initiation; Alternative splicing; Cell junction; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Neurogenesis; Phosphoprotein; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 862 AA 
Protein Sequence
MNSAEQTVTW LITLGVLESP KKTISDPEVF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 60
RNESECLSNI REFLRACGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS 120
VCARPSSHRI KSFDSLGSQS SHSRTSKLLQ SQYRSLDMTD NTNSQLVVRA KFNFQQTNED 180
ELSFSKGDVI HVTRVEEGGW WEGTHNGRTG WFPSNYVREI KPSEKPVSPK SGTLKSPPKG 240
FDTTAINKSY YNVVLQNILE TEHEYSKELQ SVLSTYLRPL QTSDKLSSAN TSYLMGNLEE 300
ISSFQQVLVQ SLEECTKSPE AQQRVGGCFL SLMPQMRTLY LAYCANHPSA VSVLTEHSED 360
LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHPDR QDIQKSMTAF 420
KNLSAQCQEV RKRKELELQI LTEPIRSWEG DDIKTLGSVT YMSQVTIQCA GSEEKNERYL 480
LLFPNLLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCT 540
SQQDLHEWVE HLQKQTKVTS VSNPTIKPHS VPSHTLPSHP LTPSSKHADS KPVALTPAYH 600
TLPHPSHHGT PHTTISWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK 660
KLLPKRKPER KPSDEEFAVR KSTAALEEDA QILKVIEAYC TSAKTRQTLN STWQGTDLMH 720
NHVLADDDQS SLDSLGRRSS LSRLEPSDLS EDSEYDSIWT AHSYRMGSAS RSRKESAPQV 780
LLPEEEKIIV EETKSNGQTV IEEKSLVDTV YALKDEVQEL RQDNKKMKKS LEEEQRARKD 840
LEKLVRKVLK NMNDPAWDET NL 862 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0030032; P:lamellipodium assembly; IMP:UniProtKB.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; IEA:Compara.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR001715; CH-domain.
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00307; CH
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2 
SMART
 SM00033; CH
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS50021; CH
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.