CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018434
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA(Ile)-lysidine synthase 
Protein Synonyms/Alias
 tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase 
Gene Name
 tilS 
Gene Synonyms/Alias
 STM0236 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
362ESVSIRFKAPGVLHIacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity). 
Sequence Annotation
 NP_BIND 21 26 ATP (Potential).  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 430 AA 
Protein Sequence
MTTLTLNTSL LSSRRILAAF SGGLDSTVLL HQLVLWRERH PDVTLRAIHI HHGLSPHADS 60
WVRHCETVCE RWQVPLVVER VTLADNGLGI EAHAREARYR AFAQTLLPGE VLATAQHLDD 120
QCETFLLALK RGSGPAGLSA MGERSPFAGT LLLRPLLRET RKTLEQWAVR HGLCWIEDES 180
NQDDAYDRNF LRLRALPLLQ QRWPHFPAAV ARSATLCAEQ ERLLDELLAS DLTDCITAEG 240
TLRLSPLMSM SDVRRAAILR RWLAMRNAPM PSRDALERIW QEVALARDDA SPCLRFGDHE 300
IRRYQSQLWW IKSVAGQHET TVAWPVWQTP LALPAGLGTV QLVPGGELRR PREEESVSIR 360
FKAPGVLHIV GRNGGRKLKK IWQEQGIPPW RRDTTPLLFY GETLIAAAGV FVTREGAAED 420
KEGVSLVWHA 430 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:HAMAP.
 GO:0006400; P:tRNA modification; IEA:HAMAP. 
Interpro
 IPR012094; Lysidine-tRNA-synth.
 IPR012796; Lysidine-tRNA-synth_C.
 IPR012795; Lysidine-tRNA-synth_N.
 IPR015262; Lysidine-tRNA-synth_subst-bd.
 IPR020825; Phe-tRNA_synthase_B3/B4.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR011063; tRNA-lysidine/thiocyt_synth. 
Pfam
 PF01171; ATP_bind_3
 PF09179; TilS
 PF11734; TilS_C 
SMART
 SM00977; TilS_C 
PROSITE
  
PRINTS