CPLM-015121

Genbank Nucleotide ID

PAX-interacting protein 1

Protein Synonyms/Alias

PAX transactivation activation domain-interacting protein

Mus musculus (Mouse)

Position	Peptide	Type	References
1003	FRKLMEHKQNKSLSE	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires Rnf8 and Ube2n. Recruits Tp53bp1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by Atm. Together with Tp53bp1 regulates Atm association. Recruits Pagr1 to sites of DNA damage and the Pagr1:Paxip1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and Rad51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as Pax2. Associates with gene promoters that are known to be regulated by Kmt2d/Mll2. During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL- containing HMT complexes (By similarity).

DOMAIN 8 93 BRCT 1.
DOMAIN 94 183 BRCT 2.
DOMAIN 588 681 BRCT 3.
DOMAIN 688 776 BRCT 4.
DOMAIN 853 934 BRCT 5.
DOMAIN 955 989 BRCT 6.
REGION 94 183 Interaction with PAGR1.
REGION 577 1056 Interaction with TP53BP1 (By similarity).
MOTIF 655 672 Nuclear localization signal (Potential).

Complete proteome; DNA damage; DNA recombination; DNA repair; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0035097; C:histone methyltransferase complex; IDA:MGI.
GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
GO:0060612; P:adipose tissue development; IMP:UniProtKB.
GO:0060717; P:chorion development; IMP:UniProtKB.
GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; IEA:Compara.
GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
GO:0043542; P:endothelial cell migration; IMP:UniProtKB.
GO:0051568; P:histone H3-K4 methylation; IEA:Compara.
GO:0035066; P:positive regulation of histone acetylation; IMP:UniProtKB.
GO:0000416; P:positive regulation of histone H3-K36 methylation; IMP:UniProtKB.
GO:0051571; P:positive regulation of histone H3-K4 methylation; IMP:UniProtKB.
GO:0045830; P:positive regulation of isotype switching; IMP:UniProtKB.
GO:0031398; P:positive regulation of protein ubiquitination; IMP:UniProtKB.
GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IMP:UniProtKB.
GO:0006974; P:response to DNA damage stimulus; IDA:MGI.
GO:0010212; P:response to ionizing radiation; IEA:Compara.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0001570; P:vasculogenesis; IMP:UniProtKB.

IPR001357; BRCT_dom.

PF00533; BRCT
PF12738; PTCB-BRCT