CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000964
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4E type 2 
Protein Synonyms/Alias
 eIF-4E type 2; eIF4E type 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E-like 3; eIF4E-like protein 4E-LP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3 
Gene Name
 EIF4E2 
Gene Synonyms/Alias
 EIF4EL3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
134DANKNGGKWIIRLRKacetylation[1]
239LLFQNLWKPRLNVP*ubiquitination[2, 3, 4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. 
Sequence Annotation
 REGION 54 57 EIF4EBP1/2/3 binding.
 REGION 78 79 7-methylguanosine-containing mRNA cap
 REGION 95 99 EIF4EBP1/2/3 binding.
 REGION 124 125 7-methylguanosine-containing mRNA cap
 REGION 150 157 EIF4EBP1/2/3 binding.
 REGION 174 179 7-methylguanosine-containing mRNA cap
 REGION 222 224 7-methylguanosine-containing mRNA cap
 BINDING 110 110 7-methylguanosine-containing mRNA cap.
 MOD_RES 134 134 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 245 AA 
Protein Sequence
MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTERDKNQS SSKRKAVVPG PAEHPLQYNY 60
TFWYSRRTPG RPTSSQSYEQ NIKQIGTFAS VEQFWRFYSH MVRPGDLTGH SDFHLFKEGI 120
KPMWEDDANK NGGKWIIRLR KGLASRCWEN LILAMLGEQF MVGEEICGAV VSVRFQEDII 180
SIWNKTASDQ ATTARIRDTL RRVLNLPPNT IMEYKTHTDS IKMPGRLGPQ RLLFQNLWKP 240
RLNVP 245 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005845; C:mRNA cap binding complex; IDA:UniProtKB.
 GO:0000339; F:RNA cap binding; TAS:ProtInc.
 GO:0008135; F:translation factor activity, nucleic acid binding; TAS:ProtInc.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0017148; P:negative regulation of translation; IMP:MGI. 
Interpro
 IPR023398; TIF_eIF4e-like_dom.
 IPR001040; TIF_eIF_4E.
 IPR019770; TIF_eIF_4E_CS. 
Pfam
 PF01652; IF4E 
SMART
  
PROSITE
 PS00813; IF4E 
PRINTS