CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001126
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Clustered mitochondria protein homolog 
Protein Synonyms/Alias
  
Gene Name
 CLUH 
Gene Synonyms/Alias
 KIAA0664 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
175GDSGKRKKGLEMDPIubiquitination[1]
210QPQNRDWKPLQCLKVubiquitination[1]
295QISPTFKKNFAVLQKubiquitination[1]
303NFAVLQKKRVQRHPFubiquitination[1]
437FDVRDHYKDFGGDVAubiquitination[1]
529ERTSRPLKILRHQVLubiquitination[1]
678ASGLAKVKELAETIAubiquitination[1, 2]
974VVKHVNPKASDAFHFubiquitination[1, 3, 4]
1159VARVYESKAEFRSALubiquitination[1]
1195KESSEYLKCLTQQAVubiquitination[1]
1257QKDLENLKAEVARRHubiquitination[5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Involved in proper cytoplasmic distribution of mitochondria (By similarity). 
Sequence Annotation
 REPEAT 978 1011 TPR 1.
 REPEAT 1020 1053 TPR 2.
 REPEAT 1104 1137 TPR 3.
 REPEAT 1146 1179 TPR 4.
 MOD_RES 279 279 Phosphoserine.
 MOD_RES 281 281 Phosphoserine.
 MOD_RES 654 654 Phosphoserine.
 MOD_RES 664 664 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1309 AA 
Protein Sequence
MLLNGDCPES LKKEAAAAEP PRENGLDEAG PGDETTGQEV IVIQDTGFSV KILAPGIEPF 60
SLQVSPQEMV QEIHQVLMDR EDTCHRTCFS LHLDGNVLDH FSELRSVEGL QEGSVLRVVE 120
EPYTVREARI HVRHVRDLLK SLDPSDAFNG VDCNSLSFLS VFTDGDLGDS GKRKKGLEMD 180
PIDCTPPEYI LPGSRERPLC PLQPQNRDWK PLQCLKVLTM SGWNPPPGNR KMHGDLMYLF 240
VITAEDRQVS ITASTRGFYL NQSTAYHFNP KPASPRFLSH SLVELLNQIS PTFKKNFAVL 300
QKKRVQRHPF ERIATPFQVY SWTAPQAEHA MDCVRAEDAY TSRLGYEEHI PGQTRDWNEE 360
LQTTRELPRK NLPERLLRER AIFKVHSDFT AAATRGAMAV IDGNVMAINP SEETKMQMFI 420
WNNIFFSLGF DVRDHYKDFG GDVAAYVAPT NDLNGVRTYN AVDVEGLYTL GTVVVDYRGY 480
RVTAQSIIPG ILERDQEQSV IYGSIDFGKT VVSHPRYLEL LERTSRPLKI LRHQVLNDRD 540
EEVELCSSVE CKGIIGNDGR HYILDLLRTF PPDLNFLPVP GEELPEECAR AGFPRAHRHK 600
LCCLRQELVD AFVEHRYLLF MKLAALQLMQ QNASQLETPS SLENGGPSSL ESKSEDPPGQ 660
EAGSEEEGSS ASGLAKVKEL AETIAADDGT DPRSREVIRN ACKAVGSISS TAFDIRFNPD 720
IFSPGVRFPE SCQDEVRDQK QLLKDAAAFL LSCQIPGLVK DCMEHAVLPV DGATLAEVMR 780
QRGINMRYLG KVLELVLRSP ARHQLDHVFK IGIGELITRS AKHIFKTYLQ GVELSGLSAA 840
ISHFLNCFLS SYPNPVAHLP ADELVSKKRN KRRKNRPPGA ADNTAWAVMT PQELWKNICQ 900
EAKNYFDFDL ECETVDQAVE TYGLQKITLL REISLKTGIQ VLLKEYSFDS RHKPAFTEED 960
VLNIFPVVKH VNPKASDAFH FFQSGQAKVQ QGFLKEGCEL INEALNLFNN VYGAMHVETC 1020
ACLRLLARLH YIMGDYAEAL SNQQKAVLMS ERVMGTEHPN TIQEYMHLAL YCFASSQLST 1080
ALSLLYRARY LMLLVFGEDH PEMALLDNNI GLVLHGVMEY DLSLRFLENA LAVSTKYHGP 1140
KALKVALSHH LVARVYESKA EFRSALQHEK EGYTIYKTQL GEDHEKTKES SEYLKCLTQQ 1200
AVALQRTMNE IYRNGSSANI PPLKFTAPSM ASVLEQLNVI NGILFIPLSQ KDLENLKAEV 1260
ARRHQLQEAS RNRDRAEEPM ATEPAPAGAP GDLGSQPPAA KDPSPSVQG 1309 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0048312; P:intracellular distribution of mitochondria; IEA:InterPro. 
Interpro
 IPR027523; CLU.
 IPR025697; CLU_dom.
 IPR023231; GSKIP/TIF31_domain.
 IPR011990; TPR-like_helical. 
Pfam
 PF13236; CLU 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS