CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016694
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase tousled-like 1 
Protein Synonyms/Alias
 Tousled-like kinase 1 
Gene Name
 Tlk1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
137QNESSQGKSIGGRGHacetylation[1]
700TEVQFPVKPVVSSEAubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly (By similarity). Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10' (By similarity). 
Sequence Annotation
 DOMAIN 456 734 Protein kinase.
 NP_BIND 462 470 ATP (By similarity).
 ACT_SITE 586 586 Proton acceptor (By similarity).
 BINDING 485 485 ATP (By similarity).
 MOD_RES 159 159 Phosphoserine (By similarity).
 MOD_RES 743 743 Phosphoserine (By similarity).  
Keyword
 ATP-binding; Cell cycle; Chromatin regulator; Coiled coil; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 766 AA 
Protein Sequence
MSVQSSSGSL EGPPSWSRLS TSPTPGSAAA ARSLLNHTPP SGRPREGAMD ELHSLDPRRQ 60
ELLEARFTGV ATGSTGSTGS CSVGAKASTN NESSNHSFGS LGSLSDKESE TPEKKQSESS 120
RGRKRKAESQ NESSQGKSIG GRGHKISDYF EYQGGNGSSP VRGIPPAIRS PQNSHSHSTP 180
SSSVRPNSPS PTALAFGDHP VVQPKQLSFK ITQTDLTMLK LAALESTKNQ DLEKKEGRID 240
DLLRANCDLR RQIDDQQKLL EKYKERLNKC ISMSKKLLIE KSTQEKLSSR EKSMQDRLRL 300
GHFTTVRHGA SFTEQWTDGF AFQNLVKQQE WVNQQREDIE RQRKLLGKRK PPTANNSQAP 360
ATNSEAKQRK TKAVNGAEND PFVRPNLPQL LTLAEYHEQE EIFKLRLGHL KKEEAEIQAE 420
LERLERVRNL HIRELKRINN EDNSQFKDHP TLNERYLLLH LLGRGGFSEV YKAFDLYEQR 480
YAAVKIHQLN KSWRDEKKEN YHKHACREYR IHKELDHPRI VKLYDYFSLD TDTFCTVLEY 540
CEGNDLDFYL KQHKLMSEKE ARSIVMQIVN ALRYLNEIKP PIIHYDLKPG NILLVDGTAC 600
GEIKITDFGL SKIMDDDSYG VDGMDLTSQG AGTYWYLPPE CFVVGKEPPK ISNKVDVWSV 660
GVIFFQCLYG RKPFGHNQSQ QDILQENTIL KATEVQFPVK PVVSSEAKAF IRRCLAYRKE 720
DRFDVHQLAN DPYLLPHMRR SNSSGNLHMS GLTATPTPPS SSIITY 766 
Gene Ontology
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; ISS:UniProtKB.
 GO:0001672; P:regulation of chromatin assembly or disassembly; ISS:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR027086; TLK. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS