CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037207
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Probable ATP-dependent RNA helicase DHX36 
Protein Synonyms/Alias
  
Gene Name
 DHX36 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22SVQAKNDKESEAQISubiquitination[1]
53NTPCSENKLDIQEKKacetylation[2]
53NTPCSENKLDIQEKKubiquitination[1, 2]
60KLDIQEKKLINQEKKubiquitination[1]
66KKLINQEKKMFRIRNubiquitination[3]
67KLINQEKKMFRIRNRubiquitination[3]
105LLEDLQKKKNDLRYIubiquitination[1]
120EMQHFREKLPSYGMQubiquitination[1]
354EEKEAIYKERWPDYVubiquitination[1]
596MELNALDKQEELTPLubiquitination[1]
731FVSSRNPKDPESNINubiquitination[1, 2, 4]
743NINSDNEKIIKAVICacetylation[5]
759GLYPKVAKIRLNLGKubiquitination[1]
772GKKRKMVKVYTKTDGubiquitination[1]
776KMVKVYTKTDGLVAVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 797 AA 
Protein Sequence
MDERREEQIV QLLNSVQAKN DKESEAQISW FAPEDHGYGT EVSTKNTPCS ENKLDIQEKK 60
LINQEKKMFR IRNRSYIDRD SEYLLQENEP DGTLDQKLLE DLQKKKNDLR YIEMQHFREK 120
LPSYGMQKEL VNLIDNHQVT VISGETGCGK TTQVTQFILD NYIERGKGSA CRIVCTQPRR 180
ISAISVAERV AAERAESCGS GNSTGYQIRL QSRLPRKQGS ILYCTTGIIL QWLQSDPYLS 240
SVSHIVLDEI HERNLQSDVL MTVVKDLLNF RSDLKVILMS ATLNAEKFSE YFGNCPMIHI 300
PGFTFPVVEY LLEDVIEKIR YVPEQKEHRS QFKRGFMQGH VNRQEKEEKE AIYKERWPDY 360
VRELRRRYSA STVDVIEMME DDKVDLNLIV ALIRYIVLEE EDGAILVFLP GWDNISTLHD 420
LLMSQVMFKS DKFLIIPLHS LMPTVNQTQV FKRTPPGVRK IVIATNIAET SITIDDVVYV 480
IDGGKIKETH FDTQNNISTM SAEWVSKANA KQRKGRAGRV QPGHCYHLYN GLRASLLDDY 540
QLPEILRTPL EELCLQIKIL RLGGIAYFLS RLMDPPSNEA VLLSIRHLME LNALDKQEEL 600
TPLGVHLARL PVEPHIGKMI LFGALFCCLD PVLTIAASLS FKDPFVIPLG KEKIADARRK 660
ELAKDTRSDH LTVVNAFEGW EEARRRGFRY EKDYCWEYFL SSNTLQMLHN MKGQFAEHLL 720
GAGFVSSRNP KDPESNINSD NEKIIKAVIC AGLYPKVAKI RLNLGKKRKM VKVYTKTDGL 780
VAVHPKSVNV EQTDFHY 797 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003725; F:double-stranded RNA binding; IEA:Compara.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0009615; P:response to virus; IEA:Compara. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS