UniProt Accession

 SPEE_MOUSE; Q64674 

Genbank Protein ID

 Z67748; L19311; BC005566 

Genbank Nucleotide ID

 CAA91561.1; AAC37666.1; AAH05566.1 

Protein Name

 Spermidine synthase 

Protein Synonyms/Alias

 SPDSY; Putrescine aminopropyltransferase 

Gene Name

 Srm 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
96	CSHPNPRKVLIIGGG	acetylation	[1]
135	DVIEVSKKFLPGMAV	acetylation	[1]
135	DVIEVSKKFLPGMAV	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine (By similarity). 

Sequence Annotation

 REGION 155 156 S-adenosylmethioninamine binding (By
 REGION 173 176 Putrescine binding (By similarity).
 ACT_SITE 173 173 Proton acceptor (By similarity).
 BINDING 49 49 S-adenosylmethioninamine (By similarity).
 BINDING 79 79 Putrescine (By similarity).
 BINDING 80 80 S-adenosylmethioninamine (By similarity).
 BINDING 104 104 S-adenosylmethioninamine (By similarity).
 BINDING 124 124 S-adenosylmethioninamine (By similarity).
 BINDING 173 173 S-adenosylmethioninamine (By similarity).
 BINDING 241 241 Putrescine (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 Acetylation; Complete proteome; Reference proteome; Spermidine biosynthesis; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 302 AA 

Protein Sequence

Gene Ontology

 GO:0004766; F:spermidine synthase activity; ISS:UniProtKB.
 GO:0008295; P:spermidine biosynthetic process; ISS:UniProtKB. 

Interpro

 IPR001045; Spermidine/spermine_synthase. 

Pfam

 PF01564; Spermine_synth 

SMART

  

PROSITE

 PS01330; SPERMIDINE_SYNTHASE_1
 PS51006; SPERMIDINE_SYNTHASE_2 

PRINTS