CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023011
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plexin-A1 
Protein Synonyms/Alias
 Semaphorin receptor NOV 
Gene Name
 PLXNA1 
Gene Synonyms/Alias
 NOV; PLXN1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
194VGTPIDGKSEYFPTLubiquitination[1]
382SCYRGEGKLSLPWLLubiquitination[1]
1041QLTNPEVKYNYTEDPubiquitination[2]
1353EVQANVEKSLTLFGQubiquitination[2]
1418LLSDLIEKNLESKNHubiquitination[3, 4]
1618SNSSTFTKSLSRYESubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down- stream signaling events in the cytoplasm (By similarity). 
Sequence Annotation
 DOMAIN 27 512 Sema.
 DOMAIN 864 959 IPT/TIG 1.
 DOMAIN 961 1045 IPT/TIG 2.
 DOMAIN 1048 1147 IPT/TIG 3.
 DOMAIN 1150 1236 IPT/TIG 4.
 MOD_RES 122 122 Phosphotyrosine (By similarity).
 CARBOHYD 77 77 N-linked (GlcNAc...).
 CARBOHYD 660 660 N-linked (GlcNAc...) (Potential).
 CARBOHYD 672 672 N-linked (GlcNAc...) (Potential).
 CARBOHYD 701 701 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1043 1043 N-linked (GlcNAc...).
 CARBOHYD 1187 1187 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1212 1212 N-linked (GlcNAc...) (Potential).
 DISULFID 95 104 By similarity.
 DISULFID 130 138 By similarity.
 DISULFID 286 407 By similarity.
 DISULFID 302 358 By similarity.
 DISULFID 376 395 By similarity.
 DISULFID 515 532 By similarity.
 DISULFID 521 563 By similarity.
 DISULFID 524 541 By similarity.
 DISULFID 535 547 By similarity.
 DISULFID 598 617 By similarity.  
Keyword
 Cell membrane; Coiled coil; Complete proteome; Disulfide bond; Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1896 AA 
Protein Sequence
MPLPPRSLQV LLLLLLLLLL LPGMWAEAGL PRAGGGSQPP FRTFSASDWG LTHLVVHEQT 60
GEVYVGAVNR IYKLSGNLTL LRAHVTGPVE DNEKCYPPPS VQSCPHGLGS TDNVNKLLLL 120
DYAANRLLAC GSASQGICQF LRLDDLFKLG EPHHRKEHYL SSVQEAGSMA GVLIAGPPGQ 180
GQAKLFVGTP IDGKSEYFPT LSSRRLMANE EDADMFGFVY QDEFVSSQLK IPSDTLSKFP 240
AFDIYYVYSF RSEQFVYYLT LQLDTQLTSP DAAGEHFFTS KIVRLCVDDP KFYSYVEFPI 300
GCEQAGVEYR LVQDAYLSRP GRALAHQLGL AEDEDVLFTV FAQGQKNRVK PPKESALCLF 360
TLRAIKEKIK ERIQSCYRGE GKLSLPWLLN KELGCINSPL QIDDDFCGQD FNQPLGGTVT 420
IEGTPLFVDK DDGLTAVAAY DYRGRTVVFA GTRSGRIRKI LVDLSNPGGR PALAYESVVA 480
QEGSPILRDL VLSPNHQYLY AMTEKQVTRV PVESCVQYTS CELCLGSRDP HCGWCVLHSI 540
CSRRDACERA DEPQRFAADL LQCVQLTVQP RNVSVTMSQV PLVLQAWNVP DLSAGVNCSF 600
EDFTESESVL EDGRIHCRSP SAREVAPITR GQGDQRVVKL YLKSKETGKK FASVDFVFYN 660
CSVHQSCLSC VNGSFPCHWC KYRHVCTHNV ADCAFLEGRV NVSEDCPQIL PSTQIYVPVG 720
VVKPITLAAR NLPQPQSGQR GYECLFHIPG SPARVTALRF NSSSLQCQNS SYSYEGNDVS 780
DLPVNLSVVW NGNFVIDNPQ NIQAHLYKCP ALRESCGLCL KADPRFECGW CVAERRCSLR 840
HHCAADTPAS WMHARHGSSR CTDPKILKLS PETGPRQGGT RLTITGENLG LRFEDVRLGV 900
RVGKVLCSPV ESEYISAEQI VCEIGDASSV RAHDALVEVC VRDCSPHYRA LSPKRFTFVT 960
PTFYRVSPSR GPLSGGTWIG IEGSHLNAGS DVAVSVGGRP CSFSWRNSRE IRCLTPPGQS 1020
PGSAPIIINI NRAQLTNPEV KYNYTEDPTI LRIDPEWSIN SGGTLLTVTG TNLATVREPR 1080
IRAKYGGIER ENGCLVYNDT TMVCRAPSVA NPVRSPPELG ERPDELGFVM DNVRSLLVLN 1140
STSFLYYPDP VLEPLSPTGL LELKPSSPLI LKGRNLLPPA PGNSRLNYTV LIGSTPCTLT 1200
VSETQLLCEA PNLTGQHKVT VRAGGFEFSP GTLQVYSDSL LTLPAIVGIG GGGGLLLLVI 1260
VAVLIAYKRK SRDADRTLKR LQLQMDNLES RVALECKEAF AELQTDIHEL TNDLDGAGIP 1320
FLDYRTYAMR VLFPGIEDHP VLKEMEVQAN VEKSLTLFGQ LLTKKHFLLT FIRTLEAQRS 1380
FSMRDRGNVA SLIMTALQGE MEYATGVLKQ LLSDLIEKNL ESKNHPKLLL RRTESVAEKM 1440
LTNWFTFLLY KFLKECAGEP LFMLYCAIKQ QMEKGPIDAI TGEARYSLSE DKLIRQQIDY 1500
KTLTLNCVNP ENENAPEVPV KGLDCDTVTQ AKEKLLDAAY KGVPYSQRPK AADMDLEWRQ 1560
GRMARIILQD EDVTTKIDND WKRLNTLAHY QVTDGSSVAL VPKQTSAYNI SNSSTFTKSL 1620
SRYESMLRTA SSPDSLRSRT PMITPDLESG TKLWHLVKNH DHLDQREGDR GSKMVSEIYL 1680
TRLLATKGTL QKFVDDLFET IFSTAHRGSA LPLAIKYMFD FLDEQADKHQ IHDADVRHTW 1740
KSNCLPLRFW VNVIKNPQFV FDIHKNSITD ACLSVVAQTF MDSCSTSEHK LGKDSPSNKL 1800
LYAKDIPNYK SWVERYYADI AKMPAISDQD MSAYLAEQSR LHLSQFNSMS ALHEIYSYIT 1860
KYKDEILAAL EKDEQARRQR LRSKLEQVVD TMALSS 1896 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0002116; C:semaphorin receptor complex; TAS:BHF-UCL.
 GO:0017154; F:semaphorin receptor activity; TAS:UniProtKB.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0060666; P:dichotomous subdivision of terminal units involved in salivary gland branching; IEA:Compara. 
Interpro
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set.
 IPR002909; IPT_TIG_rcpt.
 IPR003659; Plexin-like.
 IPR016201; Plexin-like_fold.
 IPR013548; Plexin_cytoplasmic_RasGAP_dom.
 IPR002165; Plexin_repeat.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001627; Semaphorin/CD100_Ag.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF08337; Plexin_cytopl
 PF01437; PSI
 PF01403; Sema
 PF01833; TIG 
SMART
 SM00429; IPT
 SM00423; PSI
 SM00630; Sema 
PROSITE
 PS51004; SEMA 
PRINTS