UniProt Accession

 Q8CIH9_MOUSE; Q8CIH9 

Genbank Protein ID

 AC114666; BC023841; CH466524 

Genbank Nucleotide ID

 AAH23841.1; EDL37915.1 

Protein Name

 Amidophosphoribosyltransferase 

Protein Synonyms/Alias

 ATase; Glutamine phosphoribosylpyrophosphate amidotransferase 

Gene Name

 Ppat 

Gene Synonyms/Alias

 mCG_15865 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
60	SDGSAVPKFRVHKGM	acetylation	[1, 2, 3]
60	SDGSAVPKFRVHKGM	ubiquitination	[4]
81	FTEDNLKKLYDSNLG	acetylation	[1, 3]
220	PVSDVNDKEKKSSET	ubiquitination	[4]
251	ARYCHEVKPGEIVEI	acetylation	[1]
372	RQLGVAKKFGVLSDN	acetylation	[3]
372	RQLGVAKKFGVLSDN	ubiquitination	[4]
403	NTISPIIKLLKESGA	ubiquitination	[4]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

  

Sequence Annotation

 ACT_SITE 12 12 For GATase activity (By similarity).
 METAL 280 280 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 426 426 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 503 503 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 506 506 Iron-sulfur (4Fe-4S) (By similarity).  

Keyword

 Complete proteome; Glycosyltransferase; Iron; Iron-sulfur; Magnesium; Metal-binding; Purine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 517 AA 

Protein Sequence

Gene Ontology

 GO:0004044; F:amidophosphoribosyltransferase activity; IEA:EC.
 GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0035690; P:cellular response to drug; IEA:Compara.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0006543; P:glutamine catabolic process; IEA:Compara.
 GO:0001822; P:kidney development; IEA:Compara.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
 GO:0009116; P:nucleoside metabolic process; IEA:InterPro.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro. 

Interpro

 IPR005854; Amd_phspho_trans.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR000836; PRibTrfase_dom. 

Pfam

 PF00310; GATase_2
 PF00156; Pribosyltran 

SMART

  

PROSITE

 PS51278; GATASE_TYPE_2 

PRINTS