CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002618
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nitrite reductase (NADH) large subunit 
Protein Synonyms/Alias
  
Gene Name
 nirB 
Gene Synonyms/Alias
 b3365; JW3328 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
248TGIRPRDKLATQCGLacetylation[1]
506LIRVEGIKTFEELLAacetylation[1]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
 NP_BIND 44 79 FAD (Potential).
 NP_BIND 193 225 NAD or NADP (Potential).
 METAL 641 641 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 647 647 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 681 681 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 685 685 Iron (siroheme axial ligand) (By
 METAL 685 685 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Complete proteome; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding; NAD; Nitrate assimilation; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 847 AA 
Protein Sequence
MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS SYFSHHTAEE 60
LSLVREGFYE KHGIKVLVGE RAITINRQEK VIHSSAGRTV FYDKLIMATG SYPWIPPIKG 120
SDTQDCFVYR TIEDLNAIES CARRSKRGAV VGGGLLGLEA AGALKNLGIE THVIEFAPML 180
MAEQLDQMGG EQLRRKIESM GVRVHTSKNT LEIVQEGVEA RKTMRFADGS ELEVDFIVFS 240
TGIRPRDKLA TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG ECASWNNRVF GLVAPGYKMA 300
QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD ESKEIYKRLI 360
VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL ILPAHSGSGK PSIGVDKLPD 420
SAQICSCFDV TKGDLIAAIN KGCHTVAALK AETKAGTGCG GCIPLVTQVL NAELAKQGIE 480
VNNNLCEHFA YSRQELFHLI RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI 540
LKPEHTPLQD SNDNFLANIQ KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS 600
QRLAMFGAQK DDLPEIWRQL IEAGFETGHA YAKALRMAKT CVGSTWCRYG VGDSVGLGVE 660
LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK GWNLYVCGNG GMKPRHADLL 720
AADIDRETLI KYLDRFMMFY IRTADKLTRT APWLENLEGG IDYLKAVIID DKLGLNAHLE 780
EEMARLREAV LCEWTETVNT PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV 840
TLVEDNA 847 
Gene Ontology
 GO:0009344; C:nitrite reductase complex [NAD(P)H]; IDA:EcoCyc.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoCyc.
 GO:0020037; F:heme binding; IDA:EcoCyc.
 GO:0051536; F:iron-sulfur cluster binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050661; F:NADP binding; IDA:EcoCyc.
 GO:0008942; F:nitrite reductase [NAD(P)H] activity; IDA:EcoCyc.
 GO:0009061; P:anaerobic respiration; IEP:EcoCyc.
 GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR007419; BFD-like_2Fe2S-bd_dom.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR005117; NiRdtase/SiRdtase_haem-b_fer.
 IPR012744; Nitri_red_NirB.
 IPR017121; Nitrite_Rdtase_lsu.
 IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
 IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 
Pfam
 PF04324; Fer2_BFD
 PF01077; NIR_SIR
 PF03460; NIR_SIR_ferr
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2 
SMART
  
PROSITE
 PS00365; NIR_SIR 
PRINTS
 PR00368; FADPNR.
 PR00397; SIROHAEM.