UniProt Accession

 MAGA2_HUMAN; P43356; A8K328; Q32NC6 

Genbank Protein ID

 L18920; AK290443; U82671; CH471169; BC108720; BC112158; BC112160 

Genbank Nucleotide ID

 AAA17729.1; BAF83132.1; EAW99429.1; AAI08721.1; AAI12159.1; AAI12161.1 

Protein Name

 Melanoma-associated antigen 2 

Protein Synonyms/Alias

 Cancer/testis antigen 1.2; CT1.2; MAGE-2 antigen 

Gene Name

 MAGEA2; MAGEA2B 

Gene Synonyms/Alias

 MAGE2; MAGEA2A; MAGE2; MAGEA2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
132	RAREPVTKAEMLESV	ubiquitination	[1, 2]
244	SVFAHPRKLLMQDLV	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T- lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation. 

Sequence Annotation

 DOMAIN 109 308 MAGE.  

Keyword

 Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation; Tumor antigen; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0070491; F:repressing transcription factor binding; IDA:UniProtKB.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0044257; P:cellular protein catabolic process; IMP:UniProtKB.
 GO:0090398; P:cellular senescence; IDA:UniProtKB.
 GO:1901984; P:negative regulation of protein acetylation; IDA:UniProtKB.
 GO:0033234; P:negative regulation of protein sumoylation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0051443; P:positive regulation of ubiquitin-protein ligase activity; IMP:UniProtKB.
 GO:0072331; P:signal transduction by p53 class mediator; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR002190; MAGE.
 IPR021072; Melanoma_ass_antigen_N. 

Pfam

 PF01454; MAGE
 PF12440; MAGE_N 

SMART

  

PROSITE

 PS50838; MAGE 

PRINTS