CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016273
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Osteopetrosis-associated transmembrane protein 1 
Protein Synonyms/Alias
 Chloride channel 7 beta subunit 
Gene Name
 OSTM1 
Gene Synonyms/Alias
 GL; HSPC019; UNQ6098/PRO21201 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
125LFQQVVSKMDNISRAubiquitination[1]
222KNYSEVCKNCREAYKubiquitination[2]
229KNCREAYKTLSSLYSubiquitination[2]
240SLYSEMQKMNELENKubiquitination[2]
321LILPKRLKSSTSFANubiquitination[1, 2, 3, 4, 5, 6, 7]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Required for osteoclast and melanocyte maturation and function (By similarity). 
Sequence Annotation
 MOD_RES 114 114 Phosphothreonine (By similarity).
 MOD_RES 116 116 Phosphotyrosine (By similarity).
 MOD_RES 322 322 Phosphoserine.
 MOD_RES 325 325 Phosphoserine.
 MOD_RES 333 333 Phosphoserine.
 CARBOHYD 93 93 N-linked (GlcNAc...) (Potential).
 CARBOHYD 128 128 N-linked (GlcNAc...) (Potential).
 CARBOHYD 135 135 N-linked (GlcNAc...) (Potential).
 CARBOHYD 163 163 N-linked (GlcNAc...) (Potential).
 CARBOHYD 177 177 N-linked (GlcNAc...) (Potential).
 CARBOHYD 184 184 N-linked (GlcNAc...) (Potential).
 CARBOHYD 194 194 N-linked (GlcNAc...) (Potential).
 CARBOHYD 216 216 N-linked (GlcNAc...) (Potential).
 CARBOHYD 263 263 N-linked (GlcNAc...) (Potential).
 CARBOHYD 274 274 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Glycoprotein; Lysosome; Membrane; Osteopetrosis; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 334 AA 
Protein Sequence
MEPGPTAAQR RCSLPPWLPL GLLLWSGLAL GALPFGSSPH RVFHDLLSEQ QLLEVEDLSL 60
SLLQGGGLGP LSLPPDLPDL DPECRELLLD FANSSAELTG CLVRSARPVR LCQTCYPLFQ 120
QVVSKMDNIS RAAGNTSESQ SCARSLLMAD RMQIVVILSE FFNTTWQEAN CANCLTNNSE 180
ELSNSTVYFL NLFNHTLTCF EHNLQGNAHS LLQTKNYSEV CKNCREAYKT LSSLYSEMQK 240
MNELENKAEP GTHLCIDVED AMNITRKLWS RTFNCSVPCS DTVPVIAVSV FILFLPVVFY 300
LSSFLHSEQK KRKLILPKRL KSSTSFANIQ ENSN 334 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005765; C:lysosomal membrane; TAS:Reactome.
 GO:0034220; P:ion transmembrane transport; TAS:Reactome.
 GO:0030316; P:osteoclast differentiation; IEA:Compara. 
Interpro
 IPR019172; Osteopetrosis-assoc_TM_1. 
Pfam
 PF09777; OSTMP1 
SMART
  
PROSITE
  
PRINTS