UniProt Accession

 DER_ECOLI; P0A6P5; P77254; Q8X4Y1 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC75564.2; BAA16397.2 

Protein Name

 GTPase Der 

Protein Synonyms/Alias

 Double era-like domain protein; GTP-binding protein EngA 

Gene Name

 der 

Gene Synonyms/Alias

 engA; yfgK; b2511; JW5403 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
104	PADEAIAKHLRSREK	acetylation	[1]
272	KITDAVEKFSVIKTL	acetylation	[1]
331	DGLSQEVKEQVKETL	acetylation	[1]
335	QEVKEQVKETLDFRL	acetylation	[1]
408	RGRRVKLKYAHAGGY	acetylation	[1]
428	VIHGNQVKDLPDSYK	acetylation	[1]
444	YLMNYFRKSLDVMGS	acetylation	[1]
458	SPIRIQFKEGENPYA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 GTPase that plays an essential role in the late steps of ribosome biogenesis. GTPase point mutations (but not a deletion mutant) are suppressed by mild overexpression of RelA, probably due to increased levels of the stringent response mediator (p)ppGpp. 50S subunits assembled in the absence of Der are defective and unable to assemble into 70S ribosomes. GTPase activity is stimulated by YihI. Overexpression rescues an rrmJ deletion, stabilizing the 70S ribosome. Der and RrmJ are likely to share a mechanism to stabilize 50S ribosomal subunits at a very late stage of 50S subunit maturation possibly by interacting with 23S rRNA or 23S rRNA/r-protein complex. 

Sequence Annotation

 DOMAIN 14 119 G 1.
 DOMAIN 214 322 G 2.
 DOMAIN 376 461 KH-like.
 NP_BIND 9 16 GTP 1 (Potential).
 NP_BIND 56 60 GTP 1 (Potential).
 NP_BIND 118 121 GTP 1 (Potential).
 NP_BIND 209 216 GTP 2 (Potential).
 NP_BIND 256 260 GTP 2 (Potential).
 NP_BIND 321 324 GTP 2 (Potential).  

Keyword

 Complete proteome; GTP-binding; Nucleotide-binding; Reference proteome; Repeat; Ribosome biogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 490 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:EcoCyc.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0003924; F:GTPase activity; IDA:EcoCyc.
 GO:0043022; F:ribosome binding; IDA:EcoCyc.
 GO:0000027; P:ribosomal large subunit assembly; IMP:EcoCyc. 

Interpro

 IPR016484; GTP-bd_EngA.
 IPR006073; GTP_binding_domain.
 IPR015946; KH_dom-like_a/b.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom. 

Pfam

 PF01926; MMR_HSR1 

SMART

  

PROSITE

  

PRINTS

 PR00326; GTP1OBG.