CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030653
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydrofolate reductase 
Protein Synonyms/Alias
 cDNA FLJ53008, highly similar to Dihydrofolate reductase (EC 1.5.1.3) 
Gene Name
 DHFR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MGKKTWFSIPEubiquitination[1]
12TWFSIPEKNRPLKGRubiquitination[1]
29LVLSRELKEPPQGAHubiquitination[1, 2, 3]
47RSLDDALKLTEQPELubiquitination[1, 2, 4, 5]
57EQPELANKVDMVWIVubiquitination[1, 2, 4, 5]
71VGGSSVYKEAMNHPGubiquitination[1]
81MNHPGHLKLFVTRIMubiquitination[1, 2, 3, 4, 6]
104FPEIDLEKYKLLPEYubiquitination[1, 2, 4, 5, 7]
106EIDLEKYKLLPEYPGubiquitination[1, 2, 3, 4, 5, 6]
122LSDVQEEKGIKYKFEacetylation[3, 8]
122LSDVQEEKGIKYKFEubiquitination[1]
125VQEEKGIKYKFEVYEubiquitination[1]
127EEKGIKYKFEVYEKNubiquitination[1, 2, 5]
133YKFEVYEKND*****ubiquitination[1, 2, 4, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 135 AA 
Protein Sequence
MGKKTWFSIP EKNRPLKGRI NLVLSRELKE PPQGAHFLSR SLDDALKLTE QPELANKVDM 60
VWIVGGSSVY KEAMNHPGHL KLFVTRIMQD FESDTFFPEI DLEKYKLLPE YPGVLSDVQE 120
EKGIKYKFEV YEKND 135 
Gene Ontology
 GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro. 
Interpro
 IPR024072; DHFR-like_dom.
 IPR001796; DHFR_dom. 
Pfam
 PF00186; DHFR_1 
SMART
  
PROSITE
 PS51330; DHFR_2 
PRINTS