UniProt Accession

 THIL_RAT; P17764 

Genbank Protein ID

 D00512; D13921 

Genbank Nucleotide ID

 BAA00401.1; BAA03016.1 

Protein Name

 Acetyl-CoA acetyltransferase, mitochondrial 

Protein Synonyms/Alias

 Acetoacetyl-CoA thiolase 

Gene Name

 Acat1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
63	LASQPATKLGTIAIQ	acetylation	[1]
80	IEKAGIPKEEVKEVY	acetylation	[1]
84	GIPKEEVKEVYMGNV	acetylation	[1]
171	ATPYGGVKLEDLIVK	acetylation	[1]
178	KLEDLIVKDGLTDVY	acetylation	[1]
187	GLTDVYNKIHMGNCA	acetylation	[1]
199	NCAENTAKKLSISRE	acetylation	[1]
210	ISREEQDKYAIGSYT	acetylation	[1]
220	IGSYTRSKEAWDAGK	acetylation	[1]
227	KEAWDAGKFANEITP	acetylation	[1]
242	ITISVKGKPDVVVKE	acetylation	[1]
248	GKPDVVVKEDEEYKR	acetylation	[1]
260	YKRVDFSKVPKLKTV	acetylation	[1]
263	VDFSKVPKLKTVFQK	acetylation	[1]
270	KLKTVFQKENGTVTA	acetylation	[1]
304	AAQRLKVKPLARIAA	acetylation	[1]
332	APAYAVPKVLKYAGL	acetylation	[1]
335	YAVPKVLKYAGLKKE	acetylation	[1]
340	VLKYAGLKKEDIAMW	acetylation	[1]
370	MLEIDPQKVNVHGGA	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Plays a major role in ketone body metabolism. 

Sequence Annotation

 REGION 255 257 Coenzyme A binding (By similarity).
 ACT_SITE 123 123 Acyl-thioester intermediate (By
 ACT_SITE 382 382 Proton acceptor (By similarity).
 ACT_SITE 410 410 Proton acceptor (By similarity).
 METAL 216 216 Potassium (By similarity).
 METAL 277 277 Potassium; via carbonyl oxygen (By
 METAL 278 278 Potassium; via carbonyl oxygen (By
 METAL 280 280 Potassium; via carbonyl oxygen (By
 METAL 378 378 Potassium; via carbonyl oxygen (By
 BINDING 216 216 Coenzyme A (By similarity).
 BINDING 260 260 Coenzyme A (By similarity).
 BINDING 281 281 Coenzyme A (By similarity).
 MOD_RES 171 171 N6-acetyllysine (By similarity).
 MOD_RES 178 178 N6-acetyllysine (By similarity).
 MOD_RES 187 187 N6-acetyllysine (By similarity).
 MOD_RES 227 227 N6-acetyllysine (By similarity).
 MOD_RES 248 248 N6-acetyllysine (By similarity).
 MOD_RES 260 260 N6-acetyllysine (By similarity).
 MOD_RES 335 335 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Metal-binding; Mitochondrion; Potassium; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 424 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IDA:RGD.
 GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IDA:RGD.
 GO:0050662; F:coenzyme binding; IPI:RGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0060612; P:adipose tissue development; IEP:RGD.
 GO:0007420; P:brain development; IEP:RGD.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0072229; P:metanephric proximal convoluted tubule development; IEP:RGD.
 GO:0051260; P:protein homooligomerization; IDA:RGD.
 GO:0009725; P:response to hormone stimulus; IEP:RGD.
 GO:0014070; P:response to organic cyclic compound; IEP:RGD.
 GO:0042594; P:response to starvation; IEP:RGD. 

Interpro

 IPR002155; Thiolase.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr.
 IPR020615; Thiolase_acyl_enz_int_AS.
 IPR020610; Thiolase_AS.
 IPR020617; Thiolase_C.
 IPR020613; Thiolase_CS.
 IPR020616; Thiolase_N. 

Pfam

 PF02803; Thiolase_C
 PF00108; Thiolase_N 

SMART

  

PROSITE

 PS00098; THIOLASE_1
 PS00737; THIOLASE_2
 PS00099; THIOLASE_3 

PRINTS