CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026227
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Clathrin heavy chain 1 
Protein Synonyms/Alias
  
Gene Name
 Cltc 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
43KFICIREKVGEQAQVubiquitination[1]
100QIFNIEMKSKMKAHTubiquitination[1]
193QLYSVDRKVSQPIEGubiquitination[1]
231VRGQAGGKLHIIEVGubiquitination[1]
249TGNQPFPKKAVDVFFubiquitination[1]
371AEELFARKFNALFAQacetylation[2]
371AEELFARKFNALFAQubiquitination[1]
386GNYSEAAKVAANAPKubiquitination[1]
393KVAANAPKGILRTPDubiquitination[1]
454GRKQLLEKWLKEDKLacetylation[3]
454GRKQLLEKWLKEDKLubiquitination[1]
457QLLEKWLKEDKLECSacetylation[2, 4]
457QLLEKWLKEDKLECSubiquitination[1]
460EKWLKEDKLECSEELacetylation[2]
491LRANVPNKVIQCFAEacetylation[2]
510QKIVLYAKKVGYTPDubiquitination[1]
511KIVLYAKKVGYTPDWubiquitination[1]
623HIAQLCEKAGLLQRAacetylation[2]
623HIAQLCEKAGLLQRAubiquitination[1]
641FTDLYDIKRAVVHTHacetylation[2, 3]
641FTDLYDIKRAVVHTHubiquitination[1]
741KYIQAACKTGQIKEVacetylation[4]
741KYIQAACKTGQIKEVsuccinylation[4]
802LYRNNLQKYIEIYVQacetylation[3]
802LYRNNLQKYIEIYVQubiquitination[1]
810YIEIYVQKVNPSRLPubiquitination[1]
855ELVAEVEKRNRLKLLubiquitination[1]
860VEKRNRLKLLLPWLEacetylation[2]
860VEKRNRLKLLLPWLEubiquitination[1]
885ATHNALAKIYIDSNNubiquitination[1]
911YDSRVVGKYCEKRDPubiquitination[1]
915VVGKYCEKRDPHLACubiquitination[1]
945CNENSLFKSLSRYLVubiquitination[1]
1038LLILTAIKADRTRVMacetylation[2]
1038LLILTAIKADRTRVMubiquitination[1]
1078EAFAIFRKFDVNTSAubiquitination[1]
1213GDRCYDEKMYDAAKLubiquitination[1]
1219EKMYDAAKLLYNNVSacetylation[2]
1219EKMYDAAKLLYNNVSubiquitination[1]
1258ANSTRTWKEVCFACVacetylation[2]
1258ANSTRTWKEVCFACVubiquitination[1]
1268CFACVDGKEFRLAQMacetylation[2]
1445RAVNYFSKVKQLPLVacetylation[4, 5, 6]
1445RAVNYFSKVKQLPLVsuccinylation[4]
1445RAVNYFSKVKQLPLVubiquitination[1]
1447VNYFSKVKQLPLVKPubiquitination[1]
1465SVQNHNNKSVNESLNubiquitination[1]
1613VMKEYLTKVDKLDASubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1679 AA 
Protein Sequence
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV VIIDMNDPSN 60
PIRRPISADS AIMNPASKVI ALKGIKDSGK TLQIFNIEMK SKMKAHTMTD DVTFWKWISL 120
NTVALVTDNA VYHWSMEGES QPVKMFDRHS SLAGCQIINY RTDAKQKWLL LTGISAQQNR 180
VVGAMQLYSV DRKVSQPIEG HAASFAQFKM EGNAEESTLF CFAVRGQAGG KLHIIEVGTP 240
PTGNQPFPKK AVDVFFPPEA QNDFPVAMQI SEKHDVVFLI TKYGYIHLYD LETGTCIYMN 300
RISGETIFVT APHEATAGII GVNRKGQVLS VCVEEENIIP YITNVLQNPD LALRMAVRNN 360
LAGAEELFAR KFNALFAQGN YSEAAKVAAN APKGILRTPD TIRRFQSVPA QPGQTSPLLQ 420
YFGILLDQGQ LNKYESLELC RPVLQQGRKQ LLEKWLKEDK LECSEELGDL VKSVDPTLAL 480
SVYLRANVPN KVIQCFAETG QVQKIVLYAK KVGYTPDWIF LLRNVMRISP DQGQQFAQML 540
VQDEEPLADI TQIVDVFMEY NLIQQCTAFL LDALKNNRPS EGPLQTRLLE MNLMHAPQVA 600
DAILGNQMFT HYDRAHIAQL CEKAGLLQRA LEHFTDLYDI KRAVVHTHLL NPEWLVNYFG 660
SLSVEDSLEC LRAMLSANIR QNLQICVQVA SKYHEQLSTQ SLIELFESFK SFEGLFYFLG 720
SIVNFSQDPD VHFKYIQAAC KTGQIKEVER ICRESNCYDP ERVKNFLKEA KLTDQLPLII 780
VCDRFDFVHD LVLYLYRNNL QKYIEIYVQK VNPSRLPVVI GGLLDVDCSE DVIKNLILVV 840
RGQFSTDELV AEVEKRNRLK LLLPWLEARI HEGCEEPATH NALAKIYIDS NNNPERFLRE 900
NPYYDSRVVG KYCEKRDPHL ACVAYERGQC DLELINVCNE NSLFKSLSRY LVRRKDPELW 960
GSVLLESNPY RRPLIDQVVQ TALSETQDPE EVSVTVKAFM TADLPNELIE LLEKIVLDNS 1020
VFSEHRNLQN LLILTAIKAD RTRVMEYINR LDNYDAPDIA NIAISNELFE EAFAIFRKFD 1080
VNTSAVQVLI EHIGNLDRAY EFAERCNEPA VWSQLAKAQL QKGMVKEAID SYIKADDPSS 1140
YMEVVQAANA SGNWEELVKY LQMARKKARE SYVETELIFA LAKTNRLAEL EEFINGPNNA 1200
HIQQVGDRCY DEKMYDAAKL LYNNVSNFGR LASTLVHLGE YQAAVDGARK ANSTRTWKEV 1260
CFACVDGKEF RLAQMCGLHI VVHADELEEL INYYQDRGYF EELITMLEAA LGLERAHMGM 1320
FTELAILYSK FKPQKMREHL ELFWSRVNIP KVLRAAEQAH LWAELVFLYD KYEEYDNAII 1380
TMMNHPTDAW KEGQFKDIIT KVANVELYYK AIQFYLEFKP LLLNDLLMVL SPRLDHTRAV 1440
NYFSKVKQLP LVKPYLRSVQ NHNNKSVNES LNNLFITEED YQALRTSIDA YDNFDNISLA 1500
QRLEKHELIE FRRIAAYLFK GNNRWKQSVE LCKKDSLYKD AMQYASESKD TELAEELLQW 1560
FLQEEKRECF GACLFTCYDL LRPDVVLETA WRHNIMDFAM PYFIQVMKEY LTKVDKLDAS 1620
ESLRKEEEQA TETQPIVYGQ PQLMLTAGPS VAVPPQAPFG YGYTAPPYGQ PQPGFGYSM 1679 
Gene Ontology
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0030130; C:clathrin coat of trans-Golgi network vesicle; IEA:InterPro.
 GO:0030117; C:membrane coat; IDA:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0031523; C:Myb complex; ISO:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0090307; P:spindle assembly involved in mitosis; ISO:MGI.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000547; Clathrin_H-chain/VPS_repeat.
 IPR016025; Clathrin_H-chain_link/propller.
 IPR015348; Clathrin_H-chain_linker_core.
 IPR001473; Clathrin_H-chain_propeller_N.
 IPR022365; Clathrin_H-chain_propeller_rpt.
 IPR016341; Clathrin_heavy_chain.
 IPR011990; TPR-like_helical. 
Pfam
 PF00637; Clathrin
 PF09268; Clathrin-link
 PF01394; Clathrin_propel 
SMART
 SM00299; CLH 
PROSITE
  
PRINTS