| Tag | Content |
|---|
| CPLM ID | CPLM-013457 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | tRNA wybutosine-synthesizing protein 2 homolog |
Protein Synonyms/Alias | tRNA-yW-synthesizing protein 2; Alpha-amino-alpha-carboxypropyl transferase TYW2 |
Gene Name | TRMT12 |
Gene Synonyms/Alias | TRM12; TYW2 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 96 | LPDPVPSKRAQGCSP | ubiquitination | [1] | | 298 | FGDNRKLKLSNIADR | ubiquitination | [1] | | 357 | LGVSKVEKEHWLYPQ | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Probable S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). |
Sequence Annotation | REGION 293 294 S-adenosyl-L-methionine binding (By
BINDING 218 218 S-adenosyl-L-methionine (By similarity).
BINDING 225 225 S-adenosyl-L-methionine (By similarity).
BINDING 265 265 S-adenosyl-L-methionine (By similarity). |
Keyword | Complete proteome; Methyltransferase; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 448 AA |
Protein Sequence | MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE KMPDGSVALP 60
VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG CSPAQKLCLE VSRWVEGRGV 120
KWSAELEADL PRSWQRHGNL LLLSEDCFQA KQWKNLGPEL WETVALALGV QRLAKRGRVS 180
PDGTRTPAVT LLLGDHGWVE HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD 240
LYAGIGYFTL PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS 300
NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL GVSKVEKEHW 360
LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE TRIATLLQQV HGKPWKTQIL 420
HIQPVKSYAP HVDHIVLDLE CCPCPSVG 448 |
Gene Ontology | GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. |
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