UniProt Accession

 DKSA_ECOLI; P0ABS1; P18274 

Genbank Protein ID

 M34945; U00096; AP009048; M60726 

Genbank Nucleotide ID

 AAA23687.1; AAC73256.1; BAB96722.1; AAA72978.1 

Protein Name

 RNA polymerase-binding transcription factor DksA 

Protein Synonyms/Alias

 DnaK suppressor protein 

Gene Name

 dksA 

Gene Synonyms/Alias

 b0145; JW0141 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
26	GVEPYQEKPGEEYMN	acetylation	[1]
98	RERKLIKKIEKTLKK	acetylation	[1]
101	KLIKKIEKTLKKVED	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. Also required for regulation of fis expression. Binding to RNAP disrupts interaction of RNAP with DNA, inhibits formation of initiation complexes, and amplifies effects of ppGpp and the initiating NTP on rRNA transcription. Inhibits transcript elongation, exonucleolytic RNA cleavage and pyrophosphorolysis, and increases intrinsic termination. Also involved, with RecN, in repair of DNA double-strand breaks. 

Sequence Annotation

 ZN_FING 114 138 dksA C4-type.
 METAL 114 114 Zinc.
 METAL 117 117 Zinc.
 METAL 135 135 Zinc.
 METAL 138 138 Zinc.  

Keyword

 3D-structure; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 151 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0010468; P:regulation of gene expression; IEA:HAMAP. 

Interpro

 IPR012784; DksA_RNA_pol-bd.
 IPR020460; Znf_C4-type_bac.
 IPR000962; Znf_DskA_TraR.
 IPR020458; Znf_DskA_TraR_CS. 

Pfam

 PF01258; zf-dskA_traR 

SMART

  

PROSITE

 PS01102; ZF_DKSA_1
 PS51128; ZF_DKSA_2 

PRINTS

 PR00618; DKSAZNFINGER.