UniProt Accession

 ACTN4_MOUSE; P57780 

Genbank Protein ID

 AJ289242; BC013616; BC087554 

Genbank Nucleotide ID

 CAC10069.1; AAH13616.1; AAH87554.1 

Protein Name

 Alpha-actinin-4 

Protein Synonyms/Alias

 F-actin cross-linking protein; Non-muscle alpha-actinin 4 

Gene Name

 Actn4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
126	FIASKGVKLVSIGAE	ubiquitination	[1]
215	PELIEYDKLRKDDPV	ubiquitination	[1]
218	IEYDKLRKDDPVTNL	ubiquitination	[1]
234	NAFEVAEKYLDIPKM	ubiquitination	[1]
256	NTARPDEKAIMTYVS	ubiquitination	[2]
332	TIQEMQQKLEDFRDY	ubiquitination	[1]
351	KPPKVQEKCQLEINF	ubiquitination	[1]
418	RLDHLAEKFRQKASI	ubiquitination	[1]
422	LAEKFRQKASIHEAW	ubiquitination	[1]
433	HEAWTDGKEAMLKQR	ubiquitination	[1]
451	TATLSDIKALIRKHE	ubiquitination	[1]
605	IAESNHIKLSGSNPY	acetylation	[3]
605	IAESNHIKLSGSNPY	ubiquitination	[1]
623	TPQIINSKWEKVQQL	acetylation	[3]
626	IINSKWEKVQQLVPK	acetylation	[4, 5]
626	IINSKWEKVQQLVPK	succinylation	[5]
633	KVQQLVPKRDHALLE	ubiquitination	[1]
761	QILTRDAKGISQEQM	ubiquitination	[1]
780	ASFNHFDKDHGGALG	acetylation	[3, 4, 5]
860	FKVLAGDKNFITAEE	acetylation	[5]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Glomerular epithelial cell injury associated with mutant alpha-actinin-4.
 Cybulsky AV, Takano T, Papillon J, Bijian K, Guillemette J, Kennedy CR.
 Am J Physiol Renal Physiol. 2009 Oct;297(4):F987-95. [PMID: 19640905]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). 

Sequence Annotation

 DOMAIN 1 267 Actin-binding.
 DOMAIN 51 155 CH 1.
 DOMAIN 164 267 CH 2.
 REPEAT 294 404 Spectrin 1.
 REPEAT 414 519 Spectrin 2.
 REPEAT 529 640 Spectrin 3.
 REPEAT 650 753 Spectrin 4.
 DOMAIN 766 801 EF-hand 1.
 DOMAIN 807 842 EF-hand 2.
 REGION 178 193 Polyphosphoinositide (PIP2)-binding
 MOD_RES 115 115 N6-acetyllysine (By similarity).
 MOD_RES 593 593 N6-acetyllysine (By similarity).
 MOD_RES 626 626 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Actin-binding; Calcium; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Nucleus; Protein transport; Reference proteome; Repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 912 AA 

Protein Sequence

Gene Ontology

 GO:0030863; C:cortical cytoskeleton; IDA:MGI.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0031143; C:pseudopodium; IDA:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0001725; C:stress fiber; IDA:UniProtKB.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0051015; F:actin filament binding; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0001882; F:nucleoside binding; IEA:Compara.
 GO:0042803; F:protein homodimerization activity; TAS:UniProtKB.
 GO:0051764; P:actin crosslink formation; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; IDA:UniProtKB.
 GO:0051271; P:negative regulation of cellular component movement; IDA:UniProtKB.
 GO:0051272; P:positive regulation of cellular component movement; IDA:UniProtKB.
 GO:0048549; P:positive regulation of pinocytosis; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara. 

Interpro

 IPR001589; Actinin_actin-bd_CS.
 IPR026921; Alpha-actinin.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR014837; EF-hand_Ca_insen.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 

Pfam

 PF00307; CH
 PF13405; EF_hand_4
 PF08726; efhand_Ca_insen
 PF00435; Spectrin 

SMART

 SM00033; CH
 SM00054; EFh
 SM00150; SPEC 

PROSITE

 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS