CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 5 
Protein Synonyms/Alias
 eIF-5 
Gene Name
 EIF5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55RPPTYPTKYFGCELGubiquitination[1, 2, 3]
249ILFDFVKKKKEEGVIubiquitination[2]
288TEVLFNEKIREQIKKubiquitination[4]
369YVSKELAKEIRVKAEacetylation[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
 Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). 
Sequence Annotation
 DOMAIN 233 392 W2.
 NP_BIND 27 34 GTP (Potential).
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 229 229 Phosphoserine.
 MOD_RES 389 389 Phosphoserine.
 MOD_RES 390 390 Phosphoserine.
 MOD_RES 419 419 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 431 AA 
Protein Sequence
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE 60
LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP ECENPETDLH VNPKKQTIGN 120
SCKACGYRGM LDTHHKLCTF ILKNPPENSD SGTGKKEKEK KNRKGKDKEN GSVSSSETPP 180
PPPPPNEINP PPHTMEEEED DDWGEDTTEE AQRRRMDEIS DHAKVLTLSD DLERTIEERV 240
NILFDFVKKK KEEGVIDSSD KEIVAEAERL DVKAMGPLVL TEVLFNEKIR EQIKKYRRHF 300
LRFCHNNKKA QRYLLHGLEC VVAMHQAQLI SKIPHILKEM YDADLLEEEV IISWSEKASK 360
KYVSKELAKE IRVKAEPFIK WLKEAEEESS GGEEEDEDEN IEVVYSKAAS VPKVETVKSD 420
NKDDDIDIDA I 431 
Gene Ontology
 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0003743; F:translation initiation factor activity; NAS:UniProtKB.
 GO:0006446; P:regulation of translational initiation; TAS:ProtInc.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR002735; Transl_init_fac_IF2/IF5.
 IPR016189; Transl_init_fac_IF2/IF5_N.
 IPR016190; Transl_init_fac_IF2/IF5_Zn-bd.
 IPR003307; W2_domain. 
Pfam
 PF01873; eIF-5_eIF-2B
 PF02020; W2 
SMART
 SM00653; eIF2B_5
 SM00515; eIF5C 
PROSITE
 PS51363; W2 
PRINTS