UniProt Accession

 BN3D2_HUMAN; Q7Z5W3; A8K829 

Genbank Protein ID

 AK292194; CH471111; BC053560 

Genbank Nucleotide ID

 BAF84883.1; EAW58101.1; AAH53560.1 

Protein Name

 Pre-miRNA 5'-monophosphate methyltransferase 

Protein Synonyms/Alias

 BCDIN3 domain-containing protein 

Gene Name

 BCDIN3D 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
117	DIDPVLVKRAEKECP	ubiquitination	[1]
143	FMNQRTRKVLLSSFL	ubiquitination	[2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs. 

Sequence Annotation

 DOMAIN 53 274 Bin3-type SAM.  

Keyword

 Complete proteome; Cytoplasm; Methyltransferase; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 292 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0008171; F:O-methyltransferase activity; IDA:UniProtKB.
 GO:0008173; F:RNA methyltransferase activity; IDA:UniProtKB.
 GO:0010586; P:miRNA metabolic process; IDA:UniProtKB.
 GO:2000632; P:negative regulation of pre-miRNA processing; IDA:UniProtKB. 

Interpro

 IPR010675; Bin3.
 IPR024160; BIN3_SAM-bd_dom. 

Pfam

 PF06859; Bin3 

SMART

  

PROSITE

 PS51515; BIN3_SAM 

PRINTS