CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018885
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 snRNA-activating protein complex subunit 3 
Protein Synonyms/Alias
 SNAPc subunit 3; Proximal sequence element-binding transcription factor subunit beta; PSE-binding factor subunit beta; PTF subunit beta; Small nuclear RNA-activating complex polypeptide 3; snRNA-activating protein complex 50 kDa subunit; SNAPc 50 kDa subunit 
Gene Name
 SNAPC3 
Gene Synonyms/Alias
 SNAP50 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108RAVCGLDKLKCLEDGubiquitination[1]
110VCGLDKLKCLEDGEDubiquitination[1]
244QAPEHISKDLYKSAFubiquitination[1, 2]
287SHDRGYGKFQTARMEubiquitination[1, 3, 4, 5]
345TLYPLLIKKHWLWTRubiquitination[3, 4, 5]
346LYPLLIKKHWLWTRKubiquitination[1]
395HYDSEGNKLGEFLAYubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box. 
Sequence Annotation
  
Keyword
 Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 411 AA 
Protein Sequence
MAEGSRGGPT CSGVGGRQDP VSGSGGCNFP EYELPELNTR AFHVGAFGEL WRGRLRGAGD 60
LSLREPPASA LPGSQAADSD REDAAVARDL DCSLEAAAEL RAVCGLDKLK CLEDGEDPEV 120
IPENTDLVTL GVRKRFLEHR EETITIDRAC RQETFVYEME SHAIGKKPEN SADMIEEGEL 180
ILSVNILYPV IFHKHKEHKP YQTMLVLGSQ KLTQLRDSIR CVSDLQIGGE FSNTPDQAPE 240
HISKDLYKSA FFYFEGTFYN DKRYPECRDL SRTIIEWSES HDRGYGKFQT ARMEDFTFND 300
LCIKLGFPYL YCHQGDCEHV IVITDIRLVH HDDCLDRTLY PLLIKKHWLW TRKCFVCKMY 360
TARWVTNNDS FAPEDPCFFC DVCFRMLHYD SEGNKLGEFL AYPYVDPGTF N 411 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0009301; P:snRNA transcription; TAS:ProtInc.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006383; P:transcription from RNA polymerase III promoter; TAS:Reactome. 
Interpro
 IPR022042; snRNA-activating_su3. 
Pfam
 PF12251; zf-SNAP50_C 
SMART
  
PROSITE
  
PRINTS