CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001001
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Target of Myb protein 1 
Protein Synonyms/Alias
  
Gene Name
 TOM1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
106LVRTILPKNNPPTIVubiquitination[1, 2, 3]
147IYEDLRRKGLEFPMTubiquitination[2, 4]
311FRTGQTTKAPSEAEPubiquitination[3]
382SSLADQRKEVKYEAPubiquitination[2]
385ADQRKEVKYEAPQATubiquitination[1, 2, 4]
452KFLEERAKAADRLPNubiquitination[3]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 May be involved in intracellular trafficking. Probable association with membranes. 
Sequence Annotation
 DOMAIN 20 152 VHS.
 DOMAIN 215 303 GAT.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 160 160 Phosphoserine.
 MOD_RES 164 164 Phosphothreonine.
 MOD_RES 174 174 Phosphothreonine (By similarity).
 MOD_RES 176 176 Phosphoserine (By similarity).
 MOD_RES 180 180 Phosphoserine (By similarity).
 MOD_RES 355 355 Phosphoserine.
 MOD_RES 462 462 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 492 AA 
Protein Sequence
MDFLLGNPFS SPVGQRIEKA TDGSLQSEDW ALNMEICDII NETEEGPKDA LRAVKKRIVG 60
NKNFHEVMLA LTVLETCVKN CGHRFHVLVA SQDFVESVLV RTILPKNNPP TIVHDKVLNL 120
IQSWADAFRS SPDLTGVVTI YEDLRRKGLE FPMTDLDMLS PIHTPQRTVF NSETQSGQDS 180
VGTDSSQQED SGQHAAPLPA PPILSGDTPI APTPEQIGKL RSELEMVSGN VRVMSEMLTE 240
LVPTQAEPAD LELLQELNRT CRAMQQRVLE LIPQIANEQL TEELLIVNDN LNNVFLRHER 300
FERFRTGQTT KAPSEAEPAA DLIDMGPDPA ATGNLSSQLA GMNLGSSSVR AGLQSLEASG 360
RLEDEFDMFA LTRGSSLADQ RKEVKYEAPQ ATDGLAGALD ARQQSTGAIP VTQACLMEDI 420
EQWLSTDVGN DAEEPKGVTS EEFDKFLEER AKAADRLPNL SSPSAEGPPG PPSGPAPRKK 480
TQEKDDDMLF AL 492 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0006897; P:endocytosis; NAS:UniProtKB.
 GO:0016197; P:endosomal transport; NAS:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0015031; P:protein transport; NAS:UniProtKB. 
Interpro
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR014645; TOM1.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00288; VHS 
PROSITE
 PS50909; GAT
 PS50179; VHS 
PRINTS