CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042318
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase 
Protein Synonyms/Alias
  
Gene Name
 NGLY1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
196CIPVQELKRKSQEKLubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 240 AA 
Protein Sequence
XSGSASPAVA ELCQNTPETF LEASKLLLTY ADNILRNPND EKYRSIRIGN TAFSTRLLPV 60
RGAVECLFEM GFEEGETHLI FPKKASVEQL QKIRDLIAIE RSSRLDGSNK SHKVKSSQQP 120
AASTQLPTTP SSNPSGLNQH TRNRQGQSSD PPSASTVAAD SAILEVLQSN IQHVLVYENP 180
ALQEKALACI PVQELKRKSQ EKLSRARKLD KDIITLRNFW KQDVDGVASG PIVLHCAAEL 240 
Gene Ontology
  
Interpro
 IPR018997; PUB_domain.
 IPR006567; PUG-dom. 
Pfam
 PF09409; PUB 
SMART
 SM00580; PUG 
PROSITE
  
PRINTS