| Tag | Content |
|---|
| CPLM ID | CPLM-024268 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Acetyl-coenzyme A synthetase |
Protein Synonyms/Alias | AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme |
Gene Name | acsA |
Gene Synonyms/Alias | MSMEG_6179; MSMEI_6020 |
Created Date | July 27, 2013 |
Organism | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) |
NCBI Taxa ID | 246196 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 906 | LPKTRSGKIMRRLLR | acetylation | [1] |
|
Reference | [1] Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP. Xu H, Hegde SS, Blanchard JS. Biochemistry. 2011 Jul 5;50(26):5883-92. [ PMID: 21627103] |
Functional Description | Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. |
Sequence Annotation | NP_BIND 480 483 Coenzyme A (By similarity).
NP_BIND 699 704 Substrate binding (By similarity).
REGION 1 289 Unknown.
REGION 290 940 Acetyl-coenzyme A synthetase.
REGION 480 483 Coenzyme A (By similarity).
ACT_SITE 813 813 By similarity.
METAL 833 833 Magnesium; via carbonyl oxygen (By
METAL 835 835 Magnesium; via carbonyl oxygen (By
BINDING 599 599 Coenzyme A (By similarity).
BINDING 675 675 Substrate; via amide nitrogen (By
BINDING 796 796 Substrate (By similarity).
BINDING 811 811 Substrate (By similarity).
BINDING 819 819 Coenzyme A (By similarity).
BINDING 822 822 Substrate (By similarity).
MOD_RES 906 906 N6-acetyllysine (By similarity). |
Keyword | Acetylation; ATP-binding; Complete proteome; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Signal. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 940 AA |
Protein Sequence | MCCAIWSASR APACSASQLS SSHAVRPSVV PDANPRAAPR YSPDLRIQCT SGTPRPCGSS 60
PLAIVTSRET ARATTPSAVP NGASVSNSPC ATAASTGAGA LDPPVRSSIS NSRRGPTSGS 120
VRGRPRCSSS VRSAAASACS RASACIGARC HTVTNEVAPP SSASATRIAP AVTGSGSPSA 180
DSCTAPPSST ADARAPRSAD SAAVCGQPRR LTLCRCARPS ASRASSQAAA TPGSSNSGAR 240
GSGRSVITSH ASRPLIASSP TVQRNQTTVH QQTCEGPTCV QRSVTRLTAM SNPSHAEVPS 300
AYPPPADFAA NANATGELYA EAEKDRLAFW EKQAKRLSWQ TPFTDVLDWS DAPFAKWFVG 360
GKINVAYNCV DRHVEAGNGD RVAIHWEGEP VGDARSITYA ELKDEVCKAA NALTDLGLVA 420
GDRVAIYMPM IPEAIVAMLA CARLGVMHSV VFAGFSASAL RARIEDAEAK LVITSDGQYR 480
RGKAASLKEA VDEAVADQPS VKNVLVVRRT GIDVKWTDGR DLWWDETVEQ ASTEHIPAAF 540
DSEQPLFLLY TSGTTGKPKG IVHTSGGYLT QSSYTHWNVF DVKPESDVYW CTADIGWVTG 600
HTYIVYGPLS NGVTQVVYEG TPTSPTEHRH FEVIEKYGVT IYYTAPTLIR TFMKLGHQIP 660
ASHDLSSLRL LGSVGEPINP EAWRWYREHI GGGKTPIVDT WWQTETGAIM ISPLPGVTAA 720
KPGSAMTPLP GISAKIVDDE GNQLVPGADE AEHVTGYLVL DQPWPAMLRG IWGDPQRFKD 780
TYWSRFAEQG WYFAGDGARY DSDGHIWVLG RIDDVMNVSG HRISTAEVES ALVGHAGVAE 840
AAVVGASDDT TGQAICAFVI LKASAHGGPE NMIEELRAEV AREISPIAKP REIHIVPELP 900
KTRSGKIMRR LLRDVAEGRE LGDTSTLVDP SVFEAIRASK 940 |
Gene Ontology | GO:0003987; F:acetate-CoA ligase activity; IEA:HAMAP. GO:0016208; F:AMP binding; IEA:InterPro. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0019427; P:acetyl-CoA biosynthetic process from acetate; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |