CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032113
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin light polypeptide 6 
Protein Synonyms/Alias
 cDNA FLJ56329, highly similar to Myosin light polypeptide 6 
Gene Name
 MYL6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
119FDRTGDGKILYSQCGacetylation[1, 2]
119FDRTGDGKILYSQCGubiquitination[2, 3, 4, 5, 6]
143PTNAEVLKVLGNPKSacetylation[1, 2, 7, 8]
143PTNAEVLKVLGNPKSubiquitination[2, 3, 4, 5, 6, 9, 10, 11, 12, 13]
149LKVLGNPKSDEMNVKacetylation[1]
149LKVLGNPKSDEMNVKubiquitination[2, 3, 4, 5, 6, 12]
156KSDEMNVKVLDFEHFubiquitination[3, 6, 10, 12, 13]
172PMLQTVAKNKDQGTYacetylation[8]
172PMLQTVAKNKDQGTYubiquitination[3, 4, 5, 6, 12]
174LQTVAKNKDQGTYEDacetylation[1, 2, 8]
174LQTVAKNKDQGTYEDubiquitination[2, 3, 4, 5, 6, 9, 10, 12, 13]
191EGLRVFDKEGNGTVMacetylation[7]
191EGLRVFDKEGNGTVMubiquitination[3, 4, 5, 12]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [10] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [11] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [12] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [13] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 238 AA 
Protein Sequence
MVGPRSWETG RIGDERQEET GGGRGEGRGR RQRESEDPKG WRWGWKDWGP AGKRVCSLKQ 60
EFVGQSLWVR VCGAGIETRG IGVQMLTTSL FSEQCDFTED QTAEFKEAFQ LFDRTGDGKI 120
LYSQCGDVMR ALGQNPTNAE VLKVLGNPKS DEMNVKVLDF EHFLPMLQTV AKNKDQGTYE 180
DYVEGLRVFD KEGNGTVMGA EIRHVLVTLG EKMTEEEVEM LVAGHEDSNG CINYEGKR 238 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 
Pfam
 PF13405; EF_hand_4 
SMART
 SM00054; EFh 
PROSITE
 PS50222; EF_HAND_2 
PRINTS