UniProt Accession

 SRPK2_MOUSE; O54781; Q8VCD9 

Genbank Protein ID

 AB006036; BC020178 

Genbank Nucleotide ID

 BAA24055.1; AAH20178.1 

Protein Name

 SRSF protein kinase 2 

Protein Synonyms/Alias

 SFRS protein kinase 2; Serine/arginine-rich protein-specific kinase 2; SR-protein-specific kinase 2; SRSF protein kinase 2 N-terminal; SRSF protein kinase 2 C-terminal 

Gene Name

 Srpk2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
128	LDEIKLLKCVRESDP	ubiquitination	[1]
207	DYLHSKCKIIHTDIK	ubiquitination	[1]
214	KIIHTDIKPENILMC	ubiquitination	[1]
239	AEATEWQKAGAPPPS	ubiquitination	[1]
532	GNACWVHKHFTEDIQ	acetylation	[2]
611	RHFALSGKYSREFFN	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up- regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. 

Sequence Annotation

 DOMAIN 79 681 Protein kinase.
 NP_BIND 85 93 ATP (By similarity).
 ACT_SITE 212 212 Proton acceptor (By similarity).
 BINDING 108 108 ATP (By similarity).
 MOD_RES 50 50 Phosphoserine; by CK2 (By similarity).
 MOD_RES 378 378 Phosphoserine (By similarity).
 MOD_RES 485 485 Phosphothreonine; by PKB/AKT1 (By
 MOD_RES 487 487 Phosphoserine (By similarity).
 MOD_RES 490 490 Phosphoserine (By similarity).
 MOD_RES 581 581 Phosphoserine; by CK2 (By similarity).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Differentiation; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 681 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0071889; F:14-3-3 protein binding; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0001525; P:angiogenesis; IDA:BHF-UCL.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0045071; P:negative regulation of viral genome replication; ISS:BHF-UCL.
 GO:0035063; P:nuclear speck organization; IDA:BHF-UCL.
 GO:0045787; P:positive regulation of cell cycle; IDA:BHF-UCL.
 GO:0008284; P:positive regulation of cell proliferation; IDA:BHF-UCL.
 GO:0010628; P:positive regulation of gene expression; IDA:BHF-UCL.
 GO:0043525; P:positive regulation of neuron apoptotic process; IDA:BHF-UCL.
 GO:0045070; P:positive regulation of viral genome replication; ISS:BHF-UCL.
 GO:0000245; P:spliceosomal complex assembly; IDA:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS