UniProt Accession

 CD2A2_MOUSE; Q64364; Q4U255; Q9QXC7; Q9R051 

Genbank Protein ID

 L76092; BC058190; S80650; AJ238890; U49281; U49282; AF120108 

Genbank Nucleotide ID

 AAC42080.1; AAH58190.3; AAB35770.1; CAB65598.1; AAC00053.1; AAC00054.1; AAD33245.1 

Protein Name

 Cyclin-dependent kinase inhibitor 2A, isoform 3 

Protein Synonyms/Alias

 p19ARF 

Gene Name

 Cdkn2a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
26	QERVFLVKFVRSRRP	ubiquitination	[1]

Reference

 [1] N-terminal polyubiquitination and degradation of the Arf tumor suppressor.
 Kuo ML, den Besten W, Bertwistle D, Roussel MF, Sherr CJ.
 Genes Dev. 2004 Aug 1;18(15):1862-74. [PMID: 15289458] 

Functional Description

 Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2- induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 4 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP. 

Sequence Annotation

  

Keyword

 3D-structure; Alternative splicing; Apoptosis; Cell cycle; Complete proteome; DNA-binding; Mitochondrion; Nucleus; Reference proteome; rRNA processing; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 169 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0001652; C:granular component; IDA:BHF-UCL.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IDA:MGI.
 GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0055105; F:ubiquitin-protein ligase inhibitor activity; IDA:BHF-UCL.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007569; P:cell aging; IDA:MGI.
 GO:0007050; P:cell cycle arrest; IDA:MGI.
 GO:0008544; P:epidermis development; IMP:MGI.
 GO:0030889; P:negative regulation of B cell proliferation; IMP:HGNC.
 GO:0030308; P:negative regulation of cell growth; IMP:BHF-UCL.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:MGI.
 GO:0033088; P:negative regulation of immature T cell proliferation in thymus; IMP:HGNC.
 GO:0033600; P:negative regulation of mammary gland epithelial cell proliferation; IMP:MGI.
 GO:0051444; P:negative regulation of ubiquitin-protein ligase activity; IDA:BHF-UCL.
 GO:0060058; P:positive regulation of apoptotic process involved in mammary gland involution; IMP:MGI.
 GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; IC:BHF-UCL.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IMP:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:BHF-UCL.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
 GO:0046822; P:regulation of nucleocytoplasmic transport; IGI:MGI.
 GO:0031647; P:regulation of protein stability; IMP:BHF-UCL.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
 GO:0009303; P:rRNA transcription; IGI:MGI.
 GO:0048103; P:somatic stem cell division; IMP:HGNC.
 GO:0035019; P:somatic stem cell maintenance; IGI:MGI. 

Interpro

 IPR010868; Cyclin_kinase-Inhib_2A. 

Pfam

 PF07392; P19Arf_N 

SMART

  

PROSITE

  

PRINTS