CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009205
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal RNA small subunit methyltransferase H 
Protein Synonyms/Alias
 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH 
Gene Name
 rsmH 
Gene Synonyms/Alias
 mraW; yabC; b0082; JW0080 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
65PQAIAVAKTIDDPRFacetylation[1]
181EQPMTRTKELAEVVAacetylation[1]
194VAAATPVKDKFKHPAacetylation[1]
274PMTEEQLKKLGGRQLacetylation[1]
286RQLRALGKLMPGEEEacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes. 
Sequence Annotation
 REGION 35 37 S-adenosyl-L-methionine binding (By
 BINDING 55 55 S-adenosyl-L-methionine (By similarity).
 BINDING 79 79 S-adenosyl-L-methionine; via amide
 BINDING 101 101 S-adenosyl-L-methionine (By similarity).
 BINDING 108 108 S-adenosyl-L-methionine (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 313 AA 
Protein Sequence
MMENYKHTTV LLDEAVNGLN IRPDGIYIDG TFGRGGHSRL ILSQLGEEGR LLAIDRDPQA 60
IAVAKTIDDP RFSIIHGPFS ALGEYVAERD LIGKIDGILL DLGVSSPQLD DAERGFSFMR 120
DGPLDMRMDP TRGQSAAEWL QTAEEADIAW VLKTYGEERF AKRIARAIVE RNREQPMTRT 180
KELAEVVAAA TPVKDKFKHP ATRTFQAVRI WVNSELEEIE QALKSSLNVL APGGRLSIIS 240
FHSLEDRIVK RFMRENSRGP QVPAGLPMTE EQLKKLGGRQ LRALGKLMPG EEEVAENPRA 300
RSSVLRIAER TNA 313 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0071424; F:rRNA (cytosine-N4-)-methyltransferase activity; IDA:EcoCyc.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 
Interpro
 IPR002903; RsmH.
 IPR023397; SAM-dep_MeTrfase_MraW_recog. 
Pfam
 PF01795; Methyltransf_5 
SMART
  
PROSITE
  
PRINTS